A physical map of the region encompassing the human interleukin-1[alpha], interleukin-1[beta], and interleukin-1 receptor antagonist genes
- Univ. of Sheffield (United Kingdom)
- Institute of Molecular Pathology, Vienna (Austria)
The authors have constructed a restriction map of the human genomic region containing the genes encoding the three members of the interleukin-1 (IL-1) family, IL-1[alpha], IL-1[beta], and IL-1 receptor antagonist (IL-1ra). For this purpose, pulsed-field gel electrophoresis blots were hybridized with probes derived from the three genes. The genes (IL1A, IL1B, and IL1RN, respectively) were found to map to a common restriction fragment of approximately 430 kb that is flanked by two clusters of sites for methylation-sensitive rare-cutter restriction enzymes (putative CpG islands). A likely third internal CpG island was marked by two rare-cutter sites. CpG and non-CpG-specific enzymes were used to map the three genes. Relative to one terminal CpG island, the three genes were mapped to the following intervals: IL1A was between +0 and +35 kb, IL1B was between +70 and +110 kb, and IL1RN was between +330 and +430 kb. 12 refs., 2 figs.
- OSTI ID:
- 6975251
- Journal Information:
- Genomics; (United States), Journal Name: Genomics; (United States) Vol. 19:2; ISSN GNMCEP; ISSN 0888-7543
- Country of Publication:
- United States
- Language:
- English
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