cDNA cloning of an intracellular form of the human interleukin 1 receptor antagonist associated with epithelium

Journal Article · Wed May 01 04:00:00 EDT 1991 · Proceedings of the National Academy of Sciences of the United States of America; (United States)

DOI:https://doi.org/10.1073/pnas.88.9.3681· OSTI ID:5934175

Haskill, S; Van Le, L; Morris, J; Peace, A; Sporn, S A ^[1]; Martin, G; Fong, S; Ralph, P ^[2]; Bigler, C F; Arend, W P ^[3]; Jaffe, G J ^[4]; Hammerberg, C ^[5]

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1. Univ. of North Carolina, Chapel Hill (United States)
2. Cetus Corp., Emeryville, CA (United States)
3. Univ. of Colorado, Denver (United States)
4. Duke Univ., Durham, NC (United States)
5. Univ. of Michigan, Ann Arbor (United States)

A cDNA encoding a receptor antagonist of interleukin 1 (IL-1ra), secreted from human monocytes, has recently been isolated and sequenced. The authors have identified another version of this IL-1ra, which is predominantly expressed in epithelial cells. This IL-1ra lacks a leader sequence and, thus, is probably intracellular. Both proteins are derived from the same gene through use of an alternative transcriptional start site and internal splice-acceptor site. Expression of intracellular IL-1ra cDNA in COS cells demonstrated that the intracellular product specifically inhibited exogenous interleukin 1-dependent responses. Keratinocytes were shown to contain significant amounts of nonsecreted IL-1ra protein. Constitutive expression of the intracellular IL-1ra may be an intracellular defensive mechanism in exposed epithelial cells and/or may serve to regulate autocrine interleukin 1-mediated pathways of differentiation.

• OSTI ID: 5934175
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (United States), Journal Name: Proceedings of the National Academy of Sciences of the United States of America; (United States) Vol. 88:9; ISSN 0027-8424; ISSN PNASA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMAL TISSUES
BIOASSAY
BODY
CLONING
DNA
DNA HYBRIDIZATION
DNA-CLONING
ENZYME IMMUNOASSAY
EPITHELIUM
GROWTH FACTORS
HYBRIDIZATION
IMMUNOASSAY
LYMPHOKINES
MEMBRANE PROTEINS
MITOGENS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PROSTAGLANDINS
PROTEINS
RECEPTORS
RECOMBINANT DNA
TISSUES