DNA sequence analysis of human chromosome 21 NotI linking clones

Journal Article · Mon Nov 01 04:00:00 EST 1993 · Genomics; (United States)

DOI:https://doi.org/10.1006/geno.1993.1455· OSTI ID:6975080

Zhu, Y; Cantor, C R; Smith, C L ^[1]

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1. Lawrence Berkeley Laboratory, CA (United States)

Portions of 16 chromosome 21 NotI linking clones were sequenced. These linking clone sequences represent sequence-tagged restriction sites that are potentially useful for finding genes and for finer genome mapping and sequencing. All of the clones were G+C rich (54 to 83%). CpG and GpC dinucleotide frequencies were very close to the expected values based on base composition and were very similar in 15 of the clones. Most of the NotI linking clones were derived from CpG islands, which are often associated with genes. Five NotI linking clones had a high potential for coding regions; 7 additional clones may also contain coding regions. The NotI linking clones had many short homologous regions, but no extensive homologies either with each other or with GenBank sequences. 36 refs., 4 figs., 4 tabs.

• DOE Contract Number: AC03-76SF00098
• OSTI ID: 6975080
• Journal Information: Genomics; (United States), Journal Name: Genomics; (United States) Vol. 18:2; ISSN GNMCEP; ISSN 0888-7543
• Country of Publication: United States
• Language: English

Related Subjects

550400* -- Genetics
59 BASIC BIOLOGICAL SCIENCES
CHROMOSOMES
CLONING
DETECTION
DNA HYBRIDIZATION
DNA SEQUENCING
DNA-CLONING
GENES
GENETIC MAPPING
HUMAN CHROMOSOME 21
HUMAN CHROMOSOMES
HYBRIDIZATION
MAPPING
STRUCTURAL CHEMICAL ANALYSIS