Fluorescence in situ hybridization (FISH) mapping of human chromosome 1: Cytogenetic band localization of 71 NotI linking clones on chromosome 1q25

Journal Article · Thu Sep 01 04:00:00 EDT 1994 · American Journal of Human Genetics

OSTI ID:134820

Sumegi, J; Talmadge, C G; Zhen, D K ^[1]

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1. Univ. of Nebraska Medical Center, Omaha, NE (United States); and others

Seventy-one human chromosome 1q25-qter-specific lambda clones have been isolated from NotI-linking libraries which were constructed using DNA from MCH206.1 somatic cell hybrid cells. These cells contain chromosome 1q25 translocated to chromosome Xp22 as the only human chromosomes in a mouse background. The NotI-linking clones have been mapped to cytogenetic bands. The relative order of ten NotI clones in 1q32 and 1q41 and their relation to known chromosome 1 markers have been also determined. Portions of these ten NotI-linking clones were sequenced. Most of the NotI-linking clones were derived from CpG islands, which are often associated with genes. DNA sequence homologies were searched for these ten NotI-linking clones in sequences available in GeneBank. One of the NotI clones carries sequences identical to TGF-beta 2. The NotI-linking clones described here will be useful for constructing a long-range restriction map of chromosome 1q25-qter and may contribute to the cloning of disease genes.

• OSTI ID: 134820
• Report Number(s): CONF-941009--
• Journal Information: American Journal of Human Genetics, Journal Name: American Journal of Human Genetics Journal Issue: Suppl.3 Vol. 55; ISSN AJHGAG; ISSN 0002-9297
• Country of Publication: United States
• Language: English

Related Subjects

55 BIOLOGY AND MEDICINE
BASIC STUDIES
BIOLOGICAL MARKERS
CHROMOSOMAL ABERRATIONS
DISEASES
DNA HYBRIDIZATION
DNA SEQUENCING
DNA-CLONING
FLUORESCENCE
GENES
GENETIC MAPPING
HUMAN CHROMOSOME 1
HUMAN X CHROMOSOME
HYBRIDIZATION
MICE
NUCLEOTIDES
SOMATIC CELLS