Localization of tachykinin binding sites (NK1, NK2, NK3 ligands) in the rat brain

Journal Article · Mon Feb 29 23:00:00 EST 1988 · Peptides (Fayetteville, N.Y.); (United States)

DOI:https://doi.org/10.1016/0196-9781(88)90255-0· OSTI ID:6974912

Saffroy, M; Beaujouan, J C; Torrens, Y; Besseyre, J; Bergstroem, L G; Glowinski, J

A comparative autoradiographic analysis of the distribution of tachykinin binding sites was made on brain serial sections using several ligands. (1) /sup 3/H-SP, /sup 125/I-BHSP and /sup 3/H-physalaemin labeled identical binding sites (NK1 type). (2) /sup 3/H-NKB, /sup 125/I-BHE and /sup 3/H-eledoisin also labeled identical sites (NK3 type). (3) /sup 125/I-BHNKA preferentially labeled NK3 binding sites, the distribution of /sup 125/I-BHNKA binding sites being identical to that of /sup 3/H-NKB or /sup 125/I-BHE binding sites. (4) The distributions of /sup 3/H-SP and /sup 3/H-NKB binding sites were markedly different. (5) A very low density of labeling was found with /sup 3/H-NKA or /sup 125/I-NKA, and these binding sites were distributed only in areas rich in either /sup 3/H-SP or /sup 3/H-NKB binding sites. (6) Particular efforts were made to look for the presence of tachykinin binding sites in the substantia nigra, since this structure is particularly rich in SP and NKA and contains functional tachykinin receptors of the NK1 and NK2 types as suggested by physiological studies. Confirming previous reports, low or very low labeling was observed in the substantia nigra with /sup 3/H-SP or /sup 125/I-BHSP and /sup 3/H-NKB or /sup 125/I-BHE. Similar results were found with /sup 3/H-NKA, /sup 125/I-NKA or /sup 125/I-BHNKA. In conclusion, our data do not provide evidence yet for the existence of NK2 binding sites in the rat brain.

• Research Organization: Chaire de Neuropharmacologie, INSERM U. 114, Paris (France)
• OSTI ID: 6974912
• Journal Information: Peptides (Fayetteville, N.Y.); (United States), Journal Name: Peptides (Fayetteville, N.Y.); (United States) Vol. 9:2; ISSN PPTDD
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
AUTORADIOGRAPHY
BETA DECAY RADIOISOTOPES
BIOLOGICAL LOCALIZATION
BODY
BRAIN
CENTRAL NERVOUS SYSTEM
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPES
KININS
LABELLED COMPOUNDS
LIGANDS
MAMMALS
MEMBRANE PROTEINS
NERVOUS SYSTEM
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANS
PEPTIDES
POLYPEPTIDES
PROTEINS
RADIOISOTOPES
RATS
RECEPTORS
RODENTS
TRITIUM COMPOUNDS
VERTEBRATES