Cellular localization of cerebellar muscarinic receptors: an autoradiographic analysis of weaver, reeler, Purkinje cell degeneration and staggerer mice

Journal Article · Mon Feb 01 04:00:00 EST 1988 · Brain Res. Bull.; (United States)

DOI:https://doi.org/10.1016/0361-9230(88)90174-8· OSTI ID:6974881

Neustadt, A; Frostholm, A; Rotter, A

Light microscopic autoradiography of (/sup 3/H)quinuclidinyl benzilate binding sites was used to study the distribution of muscarinic cholinergic receptors in mouse mutants which have abnormalities affecting specific cerebellar cell types. In the normal C57BL/6J mouse, binding sites were distributed throughout the cerebellar cortex, with the highest levels in the granule cell layer and deep cerebellar nuclei. Normal binding site density was observed in the cerebellum of the weaver mutant in which the majority of granule cells had degenerated. The density of (/sup 3/H)quinuclidinyl benzilate binding sites was elevated in the cortex of the reeler, despite a reduction in the number of granule cells. The concentration of binding sites was also high over the Purkinje cell masses where granule cells were largely absent. No significant reduction in cortical (/sup 3/H)quinuclidinyl benzilate binding site density was detected in the Purkinje cell degeneration mutant, in which essentially all Purkinje cells had degenerated. In contrast, receptor binding in the deep cerebellar nuclei of this mutant was significantly increased. A substantial increase in labeling was observed in the cortex and deep nuclei of the staggerer cerebellum in which a large fraction of Golgi II cells, Purkinje cells, granule cells and mossy fibers have degenerated. We discuss the possibility that the persistence of (/sup 3/H)quinuclidinyl benzilate binding sites in all four mutants may imply a non-neuronal localization for a large proportion of muscarinic receptors in the mouse cerebellar cortex.

• Research Organization: Univ. of California, Irvine (USA)
• OSTI ID: 6974881
• Journal Information: Brain Res. Bull.; (United States), Journal Name: Brain Res. Bull.; (United States) Vol. 20:2; ISSN BRBUD
• Country of Publication: United States
• Language: English

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550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMALS
AUTONOMIC NERVOUS SYSTEM AGENTS
AUTORADIOGRAPHY
BODY
BRAIN
CELL CONSTITUENTS
CELL NUCLEI
CENTRAL NERVOUS SYSTEM
CEREBELLUM
CHEMICAL REACTIONS
DECOMPOSITION
DISTRIBUTION
DRUGS
LABELLED COMPOUNDS
MAMMALS
MEMBRANE PROTEINS
MICE
MUTANTS
NERVE CELLS
NERVOUS SYSTEM
ORGANIC COMPOUNDS
ORGANS
PROTEINS
RECEPTORS
RODENTS
SOMATIC CELLS
SUBCELLULAR DISTRIBUTION
SYMPATHOMIMETICS
TRITIUM COMPOUNDS
VERTEBRATES