Topographic and zonal organization of the olivocerebellar projection in the reeler mutant mouse

Journal Article · Fri Jan 22 04:00:00 EST 1988 · J. Comp. Neurol.; (United States)

DOI:https://doi.org/10.1002/cne.902670412· OSTI ID:5297277

Blatt, G J; Eisenman, L M

The organization of the olivocerebellar projection in the homozygous reeler mouse (rl/rl) was studied with the use of microinjections of /sup 3/H-leucine in different regions of the inferior olivary complex (IO) or horseradish peroxidase conjugated with wheat germ agglutinin (WGA-HRP) into medial, intermediate, or lateral regions of the reeler cerebellum. The purpose of this investigation was to determine the pattern of termination of olivocerebellar climbing fibers (CFs) in the cerebellum via an anterograde tracing technique, and to determine the topographic organization of the olivocerebellar projection via both anterograde and retrograde methods. The inferior olive injections were made via the ventral approach to the IO to minimize diffusion into other brainstem precerebellar nuclei and thus to ensure accurate well-restricted, injection sites. Labeled CF terminals were seen in both the superficial Purkinje cell (PC) layer (normally positioned PCs) and around PCs in the granular layer and central masses (ectopic PCs). The pattern of labeling is suggestive of orthogonal organization, in that vertical columns of cells are labeled. This is especially apparent in the medial PC group, where at least three bands are identified. Within an orthogonal band, CF terminals are seen around both superficial and deep Purkinje cells. Our data indicate that olivocerebellar topography is generally similar in reeler and normal mice despite severe abnormalities in target cell position in the reeler. The medial cerebellar region receives input from the caudal two-fifths of the medial accessory olive (MAO). The intermediate PC cluster receives input from more rostral portions of all three olivary divisions, while rostral portions of MAO and PO project to the lateral cerebellum.

• Research Organization: Thomas Jefferson Univ., Philadelphia, PA (USA)
• OSTI ID: 5297277
• Journal Information: J. Comp. Neurol.; (United States), Journal Name: J. Comp. Neurol.; (United States) Vol. 267:4; ISSN JCNEA
• Country of Publication: United States
• Language: English

Related Subjects

550301* -- Cytology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AGGLUTININS
AMINO ACIDS
ANIMALS
ANTIBODIES
AUTORADIOGRAPHY
BIOELECTRICITY
BODY
BRAIN
CARBOXYLIC ACIDS
CELL CONSTITUENTS
CELL NUCLEI
CENTRAL NERVOUS SYSTEM
CEREBELLUM
ELECTRICITY
ENZYMES
LABELLED COMPOUNDS
LEUCINE
MAMMALS
MICE
MOLECULAR STRUCTURE
NERVOUS SYSTEM
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
OXIDOREDUCTASES
PEROXIDASES
RODENTS
TRITIUM COMPOUNDS
VERTEBRATES