Indirect coupling to Na sup + of p-aminohippuric acid uptake into rat renal basolateral membrane vesicles

Journal Article · Sun Nov 01 04:00:00 EST 1987 · American Journal of Physiology; (USA)

OSTI ID:6974675

Shimada, H; Moewes, B; Burckhardt, G ^[1]

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1. Max-Planck-Institut fuer Biophysik (West Germany)

Experiments with basolateral membrane vesicles prepared from rat kidney cortex were performed to study the mechanism by which p-aminohippuric acid (PAH) is taken up across the contraluminal membrane and is concentrated in proximal tubule cells. An inward Na{sup +} gradient failed to stimulate ({sup 3}H)PAH uptake compared with K{sup +} or Li{sup +} and did not cause intravesicular PAH accumulation above equilibrium distribution. In the absence of Na{sup +}, the dicarboxylates glutarate and suberate cis-inhibited and trans-stimulated ({sup 3}H)PAH uptake, indicating a common transport system. In the presence of Na{sup +}, 10 {mu}M glutarate in the incubation medium did not cis-inhibit, but rather stimulated ({sup 3}H)PAH uptake and caused PAH accumulation above equilibrium distribution (over-shoot). Li{sup +} diminished this stimulation, but was without effect on ({sup 3}H)PAH/PAH- and ({sup 3}H)PAH/glutarate exchange. The data indicate the coexistence of a Na{sup +}-sensitive transport system for dicarboxylates and a Li{sup +}-insensitive PAH/dicarboxylate exchanger in the basolateral membrane. The authors propose that dicarboxylates are cotransported with Na{sup +} into the cell and subsequently exchange for extracellular PAH at the basolateral membrane. PAH uptake is thereby indirectly coupled to Na{sup +} via the Na{sup +}/dicarboxylate cotransporter.

• OSTI ID: 6974675
• Journal Information: American Journal of Physiology; (USA), Journal Name: American Journal of Physiology; (USA) Vol. 253:5; ISSN 0002-9513; ISSN AJPHA
• Country of Publication: United States
• Language: English

Related Subjects

551001* -- Physiological Systems-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALKALI METAL COMPOUNDS
AMINO ACIDS
ANIMALS
ANIONS
BODY
CARBOXYLIC ACIDS
CHARGED PARTICLES
CHLORIDES
CHLORINE COMPOUNDS
DICARBOXYLIC ACIDS
GLUTARIC ACID
HALIDES
HALOGEN COMPOUNDS
HIPPURIC ACID
HYDROGEN COMPOUNDS
IONS
KIDNEYS
LITHIUM CHLORIDES
LITHIUM COMPOUNDS
LITHIUM HALIDES
MAMMALS
MEMBRANE PROTEINS
MEMBRANE TRANSPORT
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PHYSIOLOGY
PROTEINS
RATS
RODENTS
SODIUM CHLORIDES
SODIUM COMPOUNDS
TRITIUM COMPOUNDS
TUBULES
VERTEBRATES