K-Cl transport systems in rabbit renal basolateral membrane vesicles
The transport pathways for chloride in basolateral membrane vesicles from the rabbit renal cortex were investigated. /sup 36/Cl uptake was stimulated by the presence of potassium in the uptake media compared with sodium of N-methyl-D-glucamine. In addition, potassium (/sup 86/Rb) uptake was stimulated more by chloride than by nitrate or gluconate. Neither of these processes was further stimulated by potassium gradients plus valinomycin, suggesting the presence of an electrically neutral K-Cl cotransport system. A magnesium-induced chloride conductance was also found in the basolateral membrane vesicles. In the absence of magnesium, the chloride conductance was low; valinomycin and an inwardly directed potassium gradient did not stimulated /sup 36/Cl uptake, anthracene-9-carboxylic acid did not inhibit /sup 36/Cl uptake, and valinomycin did not stimulated chloride-dependent /sup 86/Rb uptake. However, in the presence of 1 mM magnesium, opposite results were obtained; valinomycin and an inwardly directed potassium gradient stimulated /sup 36/Cl uptake, anthracene-9-carboxylic acid inhibited /sup 36/Cl uptake, and valinomycin stimulated chloride-dependent /sup 86/Rb uptake. Therefore, an electrically neutral K-Cl cotransport and magnesium-induced chloride conductance were found in renal cortial basolateral membrane vesicles prepared from the rabbit renal cortex.
- Research Organization:
- Veterans Administration Medical Center, San Francisco (USA)
- OSTI ID:
- 6804781
- Journal Information:
- Am. J. Physiol.; (United States), Journal Name: Am. J. Physiol.; (United States) Vol. 252:5; ISSN AJPHA
- Country of Publication:
- United States
- Language:
- English
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59 BASIC BIOLOGICAL SCIENCES
ACID ANHYDRASES
ALKALI METAL ISOTOPES
ALKALINE EARTH METAL COMPOUNDS
ANIMALS
ATP-ASE
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
BODY
CARBOXYLIC ACIDS
CATIONS
CELL CONSTITUENTS
CELL MEMBRANES
CHARGED PARTICLES
CHLORINE 36
CHLORINE ISOTOPES
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
ENZYMES
GLUCONIC ACID
HYDROLASES
HYDROXY ACIDS
INTERMEDIATE MASS NUCLEI
IONS
ISOMERIC TRANSITION ISOTOPES
ISOTOPES
KIDNEYS
KINETICS
LIGHT NUCLEI
MAGNESIUM COMPOUNDS
MAMMALS
MEMBRANE TRANSPORT
MEMBRANES
MINUTES LIVING RADIOISOTOPES
NITRATES
NITROGEN COMPOUNDS
NUCLEI
ODD-ODD NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
OXYGEN COMPOUNDS
PHOSPHOHYDROLASES
RABBITS
RADIOISOTOPES
REACTION KINETICS
RUBIDIUM 86
RUBIDIUM ISOTOPES
UPTAKE
VERTEBRATES
YEARS LIVING RADIOISOTOPES