(Genetics and biochemistry of surfactant synthesis in Rhodococcus sp. H13-A)
The rationale for these studies resides is that biosurfactant synthesis occurs only when cells are grown with alkanes as sole source of carbon and energy. It is reasoned that biosurfactant synthesis is linked genetically to alkane oxidation and that the identification and characterization of the alk genes would provide information about the structural and regulator genes involved, in biosurfactant synthesis. Rhodococcus H13-A chromosomal DNA was isolated and digested with the restriction endonuclease Sau3A. The shuttle vector, pMVS301, was single site cleaved with Bgl II, phosphorylated, and the chromosomal DNA fragments agated into linearized pMVS301. The ligation mixture was used to transform competent E. coli HB101. The chromosomal DNA fragment has been cloned into pMVS301 which appears to contain genes encoding the initial oxidation of alkane. Electrophoration of Rhodococaus indicates transformation by this technique. Further studies are required to optimize conditions.
- Research Organization:
- North Carolina State Univ., Raleigh, NC (United States). Dept. of Microbiology
- Sponsoring Organization:
- DOE; USDOE, Washington, DC (United States)
- DOE Contract Number:
- FG05-88ER13967
- OSTI ID:
- 6973819
- Report Number(s):
- DOE/ER/13967-T1; ON: DE93000913
- Country of Publication:
- United States
- Language:
- English
Similar Records
[Genetics and biochemistry of surfactant synthesis in Rhodococcus sp. H13-A]. Final report
Construction of an Escherichia coli-rhodococcus shuttle vector and plasmid transformation in Rhodococcus spp
Genetics and biochemistry of surfactant synthesis in arthrobacter species H13-A: Progress report, August 1, 1987-July 31, 1988
Technical Report
·
Sat Dec 30 23:00:00 EST 1989
·
OSTI ID:10183038
Construction of an Escherichia coli-rhodococcus shuttle vector and plasmid transformation in Rhodococcus spp
Journal Article
·
Sun Jan 31 23:00:00 EST 1988
· J. Bacteriol.; (United States)
·
OSTI ID:5285224
Genetics and biochemistry of surfactant synthesis in arthrobacter species H13-A: Progress report, August 1, 1987-July 31, 1988
Technical Report
·
Thu Dec 31 23:00:00 EST 1987
·
OSTI ID:7242081
Related Subjects
02 PETROLEUM
020900 -- Petroleum-- Environmental Aspects
54 ENVIRONMENTAL SCIENCES
540120 -- Environment
Atmospheric-- Chemicals Monitoring & Transport-- (1990-)
550200 -- Biochemistry
550400* -- Genetics
59 BASIC BIOLOGICAL SCIENCES
ALKANES
BACTERIA
BIODEGRADATION
BIOLOGICAL PATHWAYS
BIOSYNTHESIS
BIOTECHNOLOGY
CELL CONSTITUENTS
CHEMICAL REACTIONS
CLONING
DECOMPOSITION
DNA HYBRIDIZATION
DNA-ASE
DNA-CLONING
DOCUMENT TYPES
ENDONUCLEASES
ENZYMES
ESTERASES
HYBRIDIZATION
HYDROCARBONS
HYDROLASES
MICROORGANISMS
ORGANIC COMPOUNDS
PHOSPHODIESTERASES
PLASMIDS
PROGRESS REPORT
PROTEINS
RHODOCOCCUS
SULFUR-OXIDIZING BACTERIA
SURFACTANTS
SYNTHESIS
TRANSPOSONS
020900 -- Petroleum-- Environmental Aspects
54 ENVIRONMENTAL SCIENCES
540120 -- Environment
Atmospheric-- Chemicals Monitoring & Transport-- (1990-)
550200 -- Biochemistry
550400* -- Genetics
59 BASIC BIOLOGICAL SCIENCES
ALKANES
BACTERIA
BIODEGRADATION
BIOLOGICAL PATHWAYS
BIOSYNTHESIS
BIOTECHNOLOGY
CELL CONSTITUENTS
CHEMICAL REACTIONS
CLONING
DECOMPOSITION
DNA HYBRIDIZATION
DNA-ASE
DNA-CLONING
DOCUMENT TYPES
ENDONUCLEASES
ENZYMES
ESTERASES
HYBRIDIZATION
HYDROCARBONS
HYDROLASES
MICROORGANISMS
ORGANIC COMPOUNDS
PHOSPHODIESTERASES
PLASMIDS
PROGRESS REPORT
PROTEINS
RHODOCOCCUS
SULFUR-OXIDIZING BACTERIA
SURFACTANTS
SYNTHESIS
TRANSPOSONS