[Genetics and biochemistry of surfactant synthesis in Rhodococcus sp. H13-A]. Final report
The rationale for these studies resides is that biosurfactant synthesis occurs only when cells are grown with alkanes as sole source of carbon and energy. It is reasoned that biosurfactant synthesis is linked genetically to alkane oxidation and that the identification and characterization of the alk genes would provide information about the structural and regulator genes involved, in biosurfactant synthesis. Rhodococcus H13-A chromosomal DNA was isolated and digested with the restriction endonuclease Sau3A. The shuttle vector, pMVS301, was single site cleaved with Bgl II, phosphorylated, and the chromosomal DNA fragments agated into linearized pMVS301. The ligation mixture was used to transform competent E. coli HB101. The chromosomal DNA fragment has been cloned into pMVS301 which appears to contain genes encoding the initial oxidation of alkane. Electrophoration of Rhodococaus indicates transformation by this technique. Further studies are required to optimize conditions.
- Research Organization:
- North Carolina State Univ., Raleigh, NC (United States). Dept. of Microbiology
- Sponsoring Organization:
- USDOE, Washington, DC (United States)
- DOE Contract Number:
- FG05-88ER13967
- OSTI ID:
- 10183038
- Report Number(s):
- DOE/ER/13967--T1; ON: DE93000913
- Country of Publication:
- United States
- Language:
- English
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