Radiolabeled antibody in tumor imaging and therapy: iodine and radiometal chelates

Thesis/Dissertation · Thu Jan 01 04:00:00 EST 1987

OSTI ID:6973341

Berg, W T.A.

The use of radiolabeled monoclonal antibody in tumor imaging and therapy was evaluated using the Rauscher murine erythroleukemia. Antibody labeled with radioiodine or with DTPA-based radiometal chelates was compared with respect to in vitro and in vivo chemical stability of isotope incorporation, tumor targeting and catabolism, and efficacy. The radiometals studied were: /sup 47/scandium, for imaging and therapy; /sup 67/gallium and /sup 111/indium for imaging; and /sup 90/yttrium and /sup 212/bismuth for therapy. Experiments using /sup 153/Gd-labeled antibody demonstrated that insufficient gadolinium could be targeted to the tumor for use as a contrast agent in magnetic resonance imaging. Labeling was efficient and resulted in 70 to 90 percent incorporation of all isotopes except /sup 67/Ga, which was only adventitiously bound to immunoglobulin. As assessed by reduction of splenomegaly, /sup 90/Y-labeled specific antibody was 2- to 3-fold more potent in tumor therapy than control immunoglobulin whereas /sup 131/I-labeled preparations were equipotent. Treatment with /sup 90/Y-labeled antibody produced mild, reversible leukopenia, whereas marrow suppression limited the administrable dose of /sup 131/I-labeled immunoglobulin. In vitro. /sup 212/Bi-labeled specific antibody again appeared 2- to 3-fold more potent in cell killing than control antibody.

• Research Organization: Johns Hopkins Univ., Baltimore, MD (USA)
• OSTI ID: 6973341
• Country of Publication: United States
• Language: English

Related Subjects

550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
550604 -- Medicine-- Unsealed Radionuclides in Therapy-- (1980-)
62 RADIOLOGY AND NUCLEAR MEDICINE
ALPHA DECAY RADIOISOTOPES
ANIMALS
ANTIBODIES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BISMUTH 212
BISMUTH ISOTOPES
DAYS LIVING RADIOISOTOPES
DIAGNOSTIC USES
ELECTRON CAPTURE RADIOISOTOPES
EVEN-ODD NUCLEI
GADOLINIUM 153
GADOLINIUM ISOTOPES
GALLIUM 67
GALLIUM ISOTOPES
HEAVY NUCLEI
HOURS LIVING RADIOISOTOPES
INDIUM 111
INDIUM ISOTOPES
INTERMEDIATE MASS NUCLEI
IODINE 131
IODINE ISOTOPES
ISOMERIC TRANSITION ISOTOPES
ISOTOPES
MAMMALS
MICE
MINUTES LIVING RADIOISOTOPES
MONOCLONAL ANTIBODIES
NUCLEI
ODD-EVEN NUCLEI
ODD-ODD NUCLEI
RADIOISOTOPES
RARE EARTH ISOTOPES
RARE EARTH NUCLEI
RODENTS
SCANDIUM 47
SCANDIUM ISOTOPES
THERAPEUTIC USES
USES
VERTEBRATES
YTTRIUM 90
YTTRIUM ISOTOPES