Stability, targeting, and biodistribution of scandium-46- and gallium-67-labeled monoclonal antibody in erythroleukemic mice

Journal Article · Wed May 01 04:00:00 EDT 1985 · Cancer Res.; (United States)

OSTI ID:5229441

Anderson, W T; Strand, M

Using a monoclonal antibody specific for the Mr 70,000 glycoprotein of Rauscher erythroleukemia virus, the authors have determined the optimal conditions for conjugation with the cyclic anhydride of diethylene triamine pentaacetic acid and subsequent labeling with /sup 46/Sc. The conjugates were shown to retain their specificity and activity in vitro and to target specifically to virus-infected spleen cells in vivo. The stability of the /sup 46/Sc:diethylene triamine pentaacetic acid-antibody conjugates in vivo was studied using immunoaffinity chromatography; 25% of the isotope bound to transferrin, and 75% remained bound to the antibody conjugates. These results are discussed with respect to the potential for labeling antibodies with /sup 47/Sc for use in imaging and therapy. Studies with /sup 111/In-labeled antibody were used for comparison. Labeling with /sup 67/Ga was also performed; these labeled conjugates showed adventitious binding of the isotope to the antibody and lack of stability of diethylene triamine pentaacetic acid-chelated gallium. Free EDTA was shown to stably incorporate /sup 67/Ga.

• Research Organization: Johns Hopkins Univ. School of Medicine, Baltimore, MD
• OSTI ID: 5229441
• Journal Information: Cancer Res.; (United States), Journal Name: Cancer Res.; (United States) Vol. 5; ISSN CNREA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ACETIC ACID
AFFINITY
AMINO ACIDS
ANIMAL CELLS
ANIMALS
ANTIBODIES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CARBOHYDRATES
CARBOXYLIC ACIDS
CHELATING AGENTS
CHEMICAL BONDS
DAYS LIVING RADIOISOTOPES
DISTRIBUTION
DRUGS
DTPA
EDTA
ELECTRON CAPTURE RADIOISOTOPES
GALLIUM 67
GALLIUM ISOTOPES
GLUCOPROTEINS
INDIUM 111
INDIUM ISOTOPES
INTERMEDIATE MASS NUCLEI
INTERNAL CONVERSION RADIOISOTOPES
ISOMERIC TRANSITION ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
LEUKEMIA VIRUSES
MAMMALS
MICE
MICROORGANISMS
MINUTES LIVING RADIOISOTOPES
MONOCARBOXYLIC ACIDS
MONOCLONAL ANTIBODIES
NUCLEI
ODD-EVEN NUCLEI
ODD-ODD NUCLEI
ONCOGENIC VIRUSES
ORGANIC ACIDS
ORGANIC COMPOUNDS
PARASITES
PROTEINS
RADIOISOTOPES
RADIOPROTECTIVE SUBSTANCES
RODENTS
SACCHARIDES
SCANDIUM 46
SCANDIUM 47
SCANDIUM ISOTOPES
SECONDS LIVING RADIOISOTOPES
SOMATIC CELLS
SPLEEN CELLS
STABILITY
TISSUE DISTRIBUTION
TRACER TECHNIQUES
VERTEBRATES
VIRUSES