Angiotensin II effect on /sup 22/Na+ transport in vascular smooth muscle cells
Journal Article
·
· J. Cardiovasc. Pharmacol.; (United States)
It is well established that angiotensin II (AII) rapidly increases free cytosolic Ca2+ in vascular smooth muscle cells (VSMCs). Several studies have indicated that the hormone also plays a role in Na+-K+ regulation of these cells. In this study, we explored the mechanism of AII effect on /sup 22/Na+ transport in cultured rat VSMCs. The /sup 22/Na+ washout from these cells was described by three exponents with exponential factors k1 greater than k2 greater than k3. In 1.8 mM Ca2+ medium, AII (10(-9)-10(-6) M) increased (in a dose response manner) the k1 value, and consequently the initial washout rate constant (kei) for the isotope. AII had no effect on kei in Ca2+-deficient medium or in the presence of ouabain. Amiloride (10(-3) M) and verapamil (10(-5) M) abolished the AII induced increase in kei. These findings are consistent with angiotensin II stimulation of an amiloride-sensitive Na+ transport, which is likely to represent the Na+/H+ antiport. In cultured VSMCs, the sustained stimulation by AII of this transport system requires the presence of extracellular Ca2+ and its influx into these cells.
- Research Organization:
- Univ. of Medicine and Dentistry of New Jersey, Newark (USA)
- OSTI ID:
- 6970715
- Journal Information:
- J. Cardiovasc. Pharmacol.; (United States), Journal Name: J. Cardiovasc. Pharmacol.; (United States) Vol. 11:2; ISSN JCPCD
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALKALI METAL COMPOUNDS
ALKALI METAL ISOTOPES
ANGIOTENSIN
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-PLUS DECAY RADIOISOTOPES
BIOLOGICAL FUNCTIONS
CARBOHYDRATES
CARDIAC GLYCOSIDES
CARDIOTONICS
CARDIOVASCULAR AGENTS
DOSE-RESPONSE RELATIONSHIPS
DRUGS
FUNCTIONS
GLOBULINS
GLYCOSIDES
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
MAMMALS
MEMBRANE TRANSPORT
MUSCLES
NUCLEI
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
OUABAIN
PROTEINS
RADIOISOTOPES
RATS
RODENTS
SODIUM 22
SODIUM COMPOUNDS
SODIUM ISOTOPES
STROPHANTHINS
TRACER TECHNIQUES
VASOCONSTRICTORS
VERTEBRATES
YEARS LIVING RADIOISOTOPES
59 BASIC BIOLOGICAL SCIENCES
ALKALI METAL COMPOUNDS
ALKALI METAL ISOTOPES
ANGIOTENSIN
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-PLUS DECAY RADIOISOTOPES
BIOLOGICAL FUNCTIONS
CARBOHYDRATES
CARDIAC GLYCOSIDES
CARDIOTONICS
CARDIOVASCULAR AGENTS
DOSE-RESPONSE RELATIONSHIPS
DRUGS
FUNCTIONS
GLOBULINS
GLYCOSIDES
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
MAMMALS
MEMBRANE TRANSPORT
MUSCLES
NUCLEI
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
OUABAIN
PROTEINS
RADIOISOTOPES
RATS
RODENTS
SODIUM 22
SODIUM COMPOUNDS
SODIUM ISOTOPES
STROPHANTHINS
TRACER TECHNIQUES
VASOCONSTRICTORS
VERTEBRATES
YEARS LIVING RADIOISOTOPES