The role of potassium and other ions in the control of aldosterone synthesis

Journal Article · Tue Jan 01 04:00:00 EST 1991 · Endocrine Research; (United States)

DOI:https://doi.org/10.1080/07435809109027199· OSTI ID:5080528

Kenyon, C J; Shepherd, R M; Fraser, R; Pediani, J D; Elder, H Y ^[1]

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1. MRC Blood Pressure Unit, Western Infirmary, Glasgow (England)

Fast and slow K+ efflux components, independently regulated by angiotensin II (AII), have been identified in bovine adrenocortical cells. The authors have further investigated the role of potassium in the control of aldosterone synthesis in two ways. Firstly, isotopic tracers, in conjunction with channel modulators, have been used to study the interrelationship of K+ and Ca2+ in the control of AII-stimulated aldosterone synthesis. Secondly, electron probe X-ray microanalysis (EPXMA) was used to quantify potassium, sodium, chlorine and phosphorous in control and AII-stimulated cells. The effects of verapamil on 43K efflux were measured at two stages during AII stimulation. During the first ten minutes of treatment, when efflux via the fast component predominates, AII and verapamil both slowed efflux and their effects were additive. If verapamil was added later, at the time when efflux by the fast component appeared exhausted and the stimulatory effect of AII on the slow efflux component was apparent, it again slowed efflux. These data suggest that verapamil prevents calcium-gated K+ channels from opening by blocking Ca2+ channels. However, verapamil had no effect on AII-stimulated calcium efflux. In addition to blocking Ca2+ channels, verapamil may directly inhibit potassium efflux. EPXMA showed a bimodal distribution of potassium concentrations in control cells. However, in cells stimulated with AII for five minutes, the mean potassium content was less than in controls and was not bimodally distributed. Sodium content was increased by AII-treatment, chlorine was lowered and phosphorus remained unchanged. The data confirm previous observations that AII inhibits Na+/K+ ATPase activity.

• OSTI ID: 5080528
• Journal Information: Endocrine Research; (United States), Journal Name: Endocrine Research; (United States) Vol. 17:1-2; ISSN ENRSE; ISSN 0743-5800
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ACID ANHYDRASES
ADRENAL GLANDS
ADRENAL HORMONES
ALDEHYDES
ALDOSTERONE
ALKALI METAL COMPOUNDS
ALKALI METAL ISOTOPES
ALKALINE EARTH METAL COMPOUNDS
ANGIOTENSIN
ANIMALS
ATP-ASE
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL EFFECTS
BIOLOGICAL FUNCTIONS
BIOSYNTHESIS
BODY
CALCIUM COMPOUNDS
CARDIOVASCULAR AGENTS
CATTLE
CHEMICAL ANALYSIS
CHLORINE COMPOUNDS
CORTICOSTEROIDS
DOMESTIC ANIMALS
DRUGS
ELECTRON MICROPROBE ANALYSIS
ENDOCRINE GLANDS
ENZYMES
GLANDS
GLOBULINS
HALOGEN COMPOUNDS
HORMONES
HOURS LIVING RADIOISOTOPES
HYDROLASES
HYDROXY COMPOUNDS
INTERMEDIATE MASS NUCLEI
ISOTOPE APPLICATIONS
ISOTOPES
KETONES
KINETICS
MAMMALS
MEMBRANE TRANSPORT
MICROANALYSIS
MINERALOCORTICOIDS
NONDESTRUCTIVE ANALYSIS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANS
PHOSPHOHYDROLASES
PHOSPHORUS COMPOUNDS
POTASSIUM 43
POTASSIUM COMPOUNDS
POTASSIUM ISOTOPES
PREGNANES
PROTEINS
RADIOISOTOPES
REACTION KINETICS
RUMINANTS
SODIUM COMPOUNDS
STEROID HORMONES
STEROIDS
SYNTHESIS
TRACER TECHNIQUES
VASOCONSTRICTORS
VASODILATORS
VERTEBRATES