Binding of selenium-75 to blood and liver cytosolic proteins in the preruminant calf

Journal Article · Mon Feb 01 04:00:00 EST 1988 · J. Dairy Sci.; (United States)

DOI:https://doi.org/10.3168/jds.S0022-0302(88)79574-0· OSTI ID:6970711

Jenkins, K J; Hidiroglou, M

Labeled selenite (75Se) administered to calves in milk replacer, containing .2 or 5 ppm Se, was rapidly absorbed with peak blood 75Se at 6 h. Gel filtration and dialysis treatment of plasma and erythrocyte hemolysates showed that initially 75Se was transported in blood as 75SeO3= or loosely bound to plasma and erythrocyte proteins. At high Se intake, albumin became a transport protein for some of the plasma 75Se, and proportionately more blood radioactivity was carried in the erythrocytes. At 72 h after dosing, most plasma 75Se was tightly bound to protein in glutathione peroxidase fraction with low peroxidase activity, possibly Se transport protein. At 72 h, distribution of 75Se in erythrocyte was 35 to 40% in glutathione peroxidase, 50% in hemoglobin, and 5% in a selenite plus selenopolypeptide fraction. Erythrocyte peroxidase activity was mostly in the glutathione peroxidase fraction (57%) and hemoglobin (38%). Molecular weight estimate for erythrocyte glutathione peroxidase was 84,200 daltons; about 90% of blood peroxidase activity was in erythrocytes. High Se intake had no marked effect on distribution of 75Se among liver cytosolic proteins. About 35% of 75Se was in glutathione peroxidase fraction, having most of the peroxidase activity, 25% in void volume, 11 to 18% in a selenite plus selenopolypeptide fraction, and small amounts in selenoproteins of about 12,000 and 50,000 daltons.

• Research Organization: Agriculture Canada, Ottawa, Ontario
• OSTI ID: 6970711
• Journal Information: J. Dairy Sci.; (United States), Journal Name: J. Dairy Sci.; (United States) Vol. 71:2; ISSN JDSCA
• Country of Publication: United States
• Language: English

Related Subjects

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560300* -- Chemicals Metabolism & Toxicology
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
BETA DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
CALVES
CARBOXYLIC ACIDS
CATTLE
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
DOMESTIC ANIMALS
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
ELEMENTS
ENZYME ACTIVITY
ENZYMES
ERYTHROCYTES
EVEN-ODD NUCLEI
GLANDS
GLOBIN
GLUTATHIONE
HEMOGLOBIN
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
INTERMEDIATE MASS NUCLEI
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LIVER
MAMMALS
MATERIALS
METABOLISM
MOLECULAR WEIGHT
NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
OXIDOREDUCTASES
PEPTIDES
PEROXIDASES
PIGMENTS
POLYPEPTIDES
PORPHYRINS
PROTEINS
RADIOISOTOPES
RADIOPROTECTIVE SUBSTANCES
REACTION KINETICS
RUMINANTS
SELENIUM
SELENIUM 75
SELENIUM ISOTOPES
SEMIMETALS
TRACER TECHNIQUES
VERTEBRATES