Properties of a Pseudomonas stutzeri outer membrane channel-forming protein (NosA) required for production of copper-containing N sub 2 O reductase

Journal Article · Fri Mar 31 23:00:00 EST 1989 · Journal of Bacteriology; (USA)

OSTI ID:6969066

Lee, H S; Ingraham, J L ^[1]; Hancock, R E.W. ^[2]

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1. Univ. of California, Davis (USA)
2. Univ. of British Columbia, Vancouver (Canada)

A protein (NosA) in the outer membrane of Pseudomonas stutzeri that is required for copper to be inserted into N{sub 2}O reductase has been extracted and purified to homogeneity. The purified protein could form channels in black lipid bilayers. Line N{sub 2}O reductase, NosA contained copper and was only made anaerobically. In contrast to N{sub 2}O reductase, its synthesis was repressed by exogenous copper (but not by Mn, Co, Ni, Zn, or Fe). Also in contrast to N{sub 2}O reductase, NosA homologs were not immunologically detectable in Pseudomonas aeruginosa, Pseudomonas mendocina, Pseudomonas alcaligenes, or other strains of P. stutzeri.

• DOE Contract Number: FG03-85ER13356
• OSTI ID: 6969066
• Journal Information: Journal of Bacteriology; (USA), Journal Name: Journal of Bacteriology; (USA) Vol. 171:4; ISSN JOBAA; ISSN 0021-9193
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
BACTERIA
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL FUNCTIONS
CHALCOGENIDES
CHEMICAL REACTIONS
COPPER
DENITRIFICATION
ELEMENTS
ENZYMES
FUNCTIONS
KINETICS
METALS
MICROORGANISMS
MOLECULAR BIOLOGY
NITROGEN COMPOUNDS
NITROGEN OXIDES
NITROUS OXIDE
ORGANIC COMPOUNDS
OXIDES
OXIDOREDUCTASES
OXYGEN COMPOUNDS
PROTEINS
PSEUDOMONAS
REACTION KINETICS
TRANSITION ELEMENTS