Properties of a Pseudomonas stutzeri outer membrane channel-forming protein (NosA) required for production of copper-containing N sub 2 O reductase

Journal Article · Sat Apr 01 00:00:00 EST 1989 · Journal of Bacteriology; (USA)

OSTI ID:6969066

Lee, H S; Ingraham, J L ^[1]; Hancock, R E.W. ^[2]

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1. Univ. of California, Davis (USA)
2. Univ. of British Columbia, Vancouver (Canada)

A protein (NosA) in the outer membrane of Pseudomonas stutzeri that is required for copper to be inserted into N{sub 2}O reductase has been extracted and purified to homogeneity. The purified protein could form channels in black lipid bilayers. Line N{sub 2}O reductase, NosA contained copper and was only made anaerobically. In contrast to N{sub 2}O reductase, its synthesis was repressed by exogenous copper (but not by Mn, Co, Ni, Zn, or Fe). Also in contrast to N{sub 2}O reductase, NosA homologs were not immunologically detectable in Pseudomonas aeruginosa, Pseudomonas mendocina, Pseudomonas alcaligenes, or other strains of P. stutzeri.

• DOE Contract Number: FG03-85ER13356
• OSTI ID: 6969066
• Journal Information: Journal of Bacteriology; (USA), Vol. 171:4; ISSN 0021-9193
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
PROTEINS
BIOLOGICAL FUNCTIONS
PSEUDOMONAS
MOLECULAR BIOLOGY
BIOCHEMICAL REACTION KINETICS
COPPER
DENITRIFICATION
NITROUS OXIDE
OXIDOREDUCTASES
BACTERIA
CHALCOGENIDES
CHEMICAL REACTIONS
ELEMENTS
ENZYMES
FUNCTIONS
KINETICS
METALS
MICROORGANISMS
NITROGEN COMPOUNDS
NITROGEN OXIDES
ORGANIC COMPOUNDS
OXIDES
OXYGEN COMPOUNDS
REACTION KINETICS
TRANSITION ELEMENTS
550200* - Biochemistry