Pseudomonas stutzeri outer membrane proteins inserts copper into N/sub 2/O reductase

Journal Article · Tue Dec 01 04:00:00 EST 1987 · J. Bacteriol.; (United States)

OSTI ID:5272528

Mokhele, K; Tang, Y J; Clark, M A; Ingraham, J L

Among a set of frameshift mutagen (ICR-191; Polysciences, Inc.)-induced mutations that confer inability to grown anaerobically with N/sub 2/O as the sole electron acceptor, one class was found that produced an inactive N/sub 2/O reductase which lacked copper. All of these mutant strains failed to produce a 61,000-M/sub r/ protein located in the outer membrane. This protein, termed NosA, seems not to be responsible for bringing copper into the cell because the mutant strains and their parent were similarly sensitive to the copper content of the growth medium and no intermediate copper concentration in the medium permitted the mutant strains (nosA) to grow anaerobically with N/sub 2/O as the sole electron acceptor. We conclude that NosA is necessary to insert copper into N/sub 2/O reductase or to maintain it there.

• Research Organization: Univ. of California, Davis
• DOE Contract Number: FG03-85ER13356
• OSTI ID: 5272528
• Journal Information: J. Bacteriol.; (United States), Journal Name: J. Bacteriol.; (United States) Vol. 169:12; ISSN JOBAA
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
ANAEROBIC CONDITIONS
BACTERIA
BIOLOGICAL PATHWAYS
CATALYTIC EFFECTS
CELL CONSTITUENTS
CELL MEMBRANES
CHALCOGENIDES
CHEMICAL REACTIONS
COPPER
DENITRIFICATION
ELEMENTS
ENZYME ACTIVITY
ENZYMES
MEMBRANES
METALS
MICROORGANISMS
MUTANTS
NITROGEN COMPOUNDS
NITROGEN OXIDES
NITROUS OXIDE
ORGANIC COMPOUNDS
OXIDES
OXIDOREDUCTASES
OXYGEN COMPOUNDS
PROTEINS
PSEUDOMONAS
TRANSITION ELEMENTS