Continuous ethanol fermentation of lactose by a recombinant flocculating Saccharomyces cerevisiae strain
- Universidade do Minho, Braga (Portugal). Centro do Engeharia Biologica-IBQF
Alcohol fermentation of lactose was investigated using a recombinant flocculating Saccharomyces cetevisiae, expressing the LAC4 (coding the {beta}-galactosidase) and LAC12 (coding for lactose permease) genes of Kluyveromyces marxianus. Data on yeast fermentation and growth on a medium containing lactose as the sole carbon source are presented. In the range of studied lactose concentrations, total lactose consumption was observed with a conversion yield of ethanol close to the expected theoretical value. For the continuously operating bioreactor, an ethanol productivity of 11 g L{sup {minus}1} h{sup {minus}1} (corresponding to a feed lactose concentration of 50 g L{sup {minus}1} and a dilution rate of 0.55 h{sup {minus}1}) was obtained, which is 7 times larger than the continuous conventional systems. The system stability was confirmed by keeping it in operation for 6 months.
- OSTI ID:
- 696813
- Journal Information:
- Biotechnology and Bioengineering, Vol. 64, Issue 6; Other Information: PBD: 20 Sep 1999
- Country of Publication:
- United States
- Language:
- English
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