Comparison between immobilized Kluyveromyces fragilis and saccharomyces cerevisiae coimmobilized with. beta. -galactosidase, with respect to continuous ethanol production from concentrated whey permeate

Journal Article · Sat Jun 01 04:00:00 EDT 1985 · Biotechnol. Bioeng.; (United States)

DOI:https://doi.org/10.1002/bit.260270627· OSTI ID:5351558

Hahn-Haegerdal, B

Kluyveromyces fragilis immobilized in calcium alginate gel was compared to Saccharomyces cerevisiae coimmobilized with ..beta..-galactosidase, for continuous ethanol production from whey permeate in packed-bed-type columns. Four different whey concentrations were studied, equivalent to 4.5, 10, 15 and 20% lactose, respectively. In all cases the coimmobilized preparation produced more ethanol than Kluyveromyces fragilis. The study went on for more than 5 weeks. Kluyveromyces fragilis showed a decline in activity after 20 days, while the coimmobilized preparation was stable during the entire investigation. Under experimental conditions theoretical yields of ethanol were obtained from 4.5 and 10% lactose substrates with the coimmobilized system. Using 15% lactose substrate, theoretical yields were only obtained when a galactose-adapted immobilized Saccharomyces cerevisiae column was run in series with the coimmobilized column. Then a maximum of 71 g/l ethanol was produced with a productivity of 2.5 g/l hour. The coimmobilized column alone gave a maximum ethanol concentration of 52 g/l with a productivity of 4.5 g/l hour, whereas immobilized Kluyveromyces fragilis only produced 13 g/l ethanol with a productivity of 1.1 g/l hour. It was not possible to obtain theoretical yields of ethanol from the highest substrate concentration. 13 references.

• Research Organization: Univ. of Lund, Sweden
• OSTI ID: 5351558
• Journal Information: Biotechnol. Bioeng.; (United States), Journal Name: Biotechnol. Bioeng.; (United States) Vol. 27:6; ISSN BIBIA
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
ALCOHOLS
BIOCONVERSION
BIOREACTORS
CARBOHYDRATES
COMPARATIVE EVALUATIONS
CONTINUOUS CULTURE
DISACCHARIDES
ENZYME ACTIVITY
ENZYMES
ETHANOL
FERMENTATION
FUNGI
GALACTOSIDASE
GLYCOSYL HYDROLASES
HYDROLASES
HYDROXY COMPOUNDS
IMMOBILIZED CELLS
IMMOBILIZED ENZYMES
INDUSTRIAL WASTES
LACTOSE
MICROORGANISMS
O-GLYCOSYL HYDROLASES
OLIGOSACCHARIDES
ORGANIC COMPOUNDS
PACKED BED
PERFORMANCE TESTING
PLANTS
PRODUCTION
SACCHARIDES
SACCHAROMYCES
SACCHAROMYCES CEREVISIAE
TESTING
WASTES
WHEY
YEASTS