Electron autoradiographic study of intracellular conversion of fatty acids into glycogen in rats with alloxan diabetes

Journal Article · Wed May 01 04:00:00 EDT 1985 · Bull. Exp. Biol. Med. (Engl. Transl.); (United States)

OSTI ID:6966621

Lebkova, N P; Bobkov, Y I; Gorbonova, V D; Kolesova, O E

An electron-autoradiographic study was undertaken of the intracellular distribution of hydrogen of fatty acids in alloxan diabetes. Alloxan diabetes was induced in rats; between 2 weeks and 2 months after development of the disease 0.1 ml of tritium-oleic or tritium-arachidonic acid was injected into the caudel vein of the rats. After decapitation, myocardial tissue from the subendocardial zone of the left ventricle, liver tissue, and glycogen isolated from the liver by a biochemical method, were taken for electron-autoradiographic investigation. Analysis of the data showed that a radioactive isotope, injected into the blood stream of the animals in the form of oleic or arachidonic acids, is incorporated into various structures of hepatocytes and cardiomyocytes. Direct proof is obtained to show that glycogen in hepatocytes and cardiomyoctyes of diabetic rats may be formed from fatty acids.

• Research Organization: Central Postgraduate Medical Institute, Moscow
• OSTI ID: 6966621
• Journal Information: Bull. Exp. Biol. Med. (Engl. Transl.); (United States), Journal Name: Bull. Exp. Biol. Med. (Engl. Transl.); (United States) Vol. 98:12; ISSN BEXBA
• Country of Publication: United States
• Language: English

Related Subjects

550201 -- Biochemistry-- Tracer Techniques
550301* -- Cytology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALLOXAN
ANIMAL CELLS
ANIMAL TISSUES
ANIMALS
ARACHIDONIC ACID
AUTORADIOGRAPHY
AZINES
BIOCONVERSION
BIOSYNTHESIS
BODY
CARBOHYDRATES
CARBOXYLIC ACIDS
CARDIOVASCULAR SYSTEM
DIABETES MELLITUS
DISEASES
ENDOCRINE DISEASES
GLYCOGEN
HEART
HETEROCYCLIC COMPOUNDS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LIVER CELLS
MAMMALS
METABOLIC DISEASES
MONOCARBOXYLIC ACIDS
MUSCLES
MYOCARDIUM
OLEIC ACID
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
ORGANS
POLYSACCHARIDES
PYRIMIDINES
RADIORECEPTOR ASSAY
RATS
RODENTS
SACCHARIDES
SOMATIC CELLS
SYNTHESIS
TISSUES
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VERTEBRATES