IgA-binding factor suppresses synthesis of IgA in MOPC-315 plasmacytoma cells

Conference · Wed Mar 05 04:00:00 EST 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:6966041

Darby, C; Moore, J S; Muller, S; Aaronsen, D; Madianos, E; Hoover, R G

T cells with Fc receptors for IgA (T/sup ..cap alpha../ cells) and their products, IgA-binding factors (IgABF), have been implicated in the regulation of IgA expression by B cells. They have previously shown that an IgABF produced by IgA induced normal T cells or constitutively by the Fc/sup ..cap alpha../ R+ T cell lymphoma, BALENTL 8, is capable of suppressing the proliferation and the amount of secreted IgA by MOPC-315 cells. In the present studies, they demonstrate that: (a) suppression of proliferation and secretion requires surface membrane IgA on the target cell, (b) suppression exhibits rapid kinetics with maximal effect occurring by 3-4 hours, (c) suppression is reversible, and (d) suppression of secretion involves selective suppression of IgA synthesis as measured by /sup 3/H-leucine incorporation into immunoprecipitable IgA(non-IgA protein is unaffected). These findings indicate that IgA-isotype-specific effector molecules interact directly with their B cell targets through surface membrane immunoglobulin and cause a down regulation of immunoglobulin synthesis by the target. Current studies are underway to address whether this selective suppression of IgA is mediated at the transcriptional, translational or post-translational level. The use of MOPC-315 tumor cells as targets of T cell produced, isotype-specific, effector molecules should provide a unique model for the further analysis of isotype regulation at the molecular level.

• Research Organization: Univ. of Pennsylvania School of Medicine, Philadelphia
• OSTI ID: 6966041
• Report Number(s): CONF-8604222-
• Conference Information: Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Journal Volume: 45:4
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
ANIMAL CELLS
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL MATERIALS
BIOLOGICAL MODELS
BIOSYNTHESIS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARBOXYLIC ACIDS
CELL PROLIFERATION
CONNECTIVE TISSUE CELLS
GLOBULINS
IMMUNOGLOBULINS
IMMUNOSUPPRESSION
ISOTOPE APPLICATIONS
KINETICS
LABELLED COMPOUNDS
LEUCINE
LEUKOCYTES
LYMPHOCYTES
MATERIALS
MEMBRANE PROTEINS
ORGANIC ACIDS
ORGANIC COMPOUNDS
PROTEINS
REACTION KINETICS
RECEPTORS
SOMATIC CELLS
SYNTHESIS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
TUMOR CELLS