Studies on the mechanisms of TNP-LPS-induced enhancement of MOPC-315 cell secretory differentiation

Conference · Wed Mar 05 04:00:00 EST 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:7024782

Rohrer, J W

The author previously reported that incubation of MOPC-315 cells in vitro for 48 hours with 0.01 ..mu..g/ml of TNP-LPS induced enhanced MOPC-315 cell secretory differentiation. He now reports that MOPC-315 cells become committed to enhanced secretory differentiation during the first 2 hours of TNP-LPS exposure. This commitment can be blocked by including the phospholipid methylation inhibitor 2-hydroxyethylhydrazine (2-HEH) in the TNP-LPS culture during the first 30 minutes. Inclusion of the serine protease inhibitor diisopropylfluorophosphate (DFP) in the TNP-LPS culture for the first 2 hours also blocks commitment to enhanced 315 cell PFC frequency. A serine protease is activated by the TNP-LPS exposure since 315 cells cultured with DFP before, but not during, TNP-LPS culture still show normal TNP-LPS enhancement. Phospholipid methylation is required for serine protease activation since incubation of the cells with 2-HEH for 30 minutes blocks the enhanced accumulation of /sup 3/H-DFP usually seen at 2 hours of TNP-LPS culture. The TNP-LPS-induced serine protease is specific for lysine since lysine analogs, but not other amino acid analogs, block TNP-LPS-induced enhancement. Protein kinase C is not required for this enhancement since inclusion of the protein kinase C inhibitor, into the TNP-LPS culture does not inhibit the enhancement of MOPC-315 PFC frequency.

• Research Organization: Univ. of South Alabama College of Medicine, Mobile
• OSTI ID: 7024782
• Report Number(s): CONF-8604222-
• Conference Information: Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Journal Volume: 45:4
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CELL DIFFERENTIATION
CONNECTIVE TISSUE CELLS
ENZYME ACTIVITY
ENZYME INHIBITORS
ENZYMES
HYDROLASES
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LEUKOCYTES
LYMPHOCYTES
MATERIALS
PEPTIDE HYDROLASES
SERINE PROTEINASES
SOMATIC CELLS
TRACER TECHNIQUES
TRITIUM COMPOUNDS