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Characterization of an oligopeptide chemoattractant receptor on human blood monocytes using a new radioligand

Journal Article · · Blood; (United States)
OSTI ID:6965659
;
The study of chemoattractant receptors on human monocytes had been limited by the lack of a radioligand suitable for use with the small numbers of cells routinely available from human donors. A new synthetic oligopeptide radioligand f(/sup 35/S)Met-leu-phe, with a higher specific radioactivity than was available with the tritiated compound, was used to characterize a chemoattractant receptor on freshly isolated human blood monocytes. These cells bind f(/sup 35/)met-leu-phe with a dissociation constant (KD) of 30.2 +/- 5.6 nM and contain 84,000 +/- 11,300 receptors per cell. f(/sup 35/)met-leu-phe does not bind specifically to blood lymphocytes. The specificity of the oligopeptide receptor on monocytes is indistinguishable from the oligopeptide chemoattractant receptor on human polymorphonuclear leukocytes. Using f(/sup 35/)met-leu-phe, it will now be feasible to study the chemotactic peptide receptor on small numbers of partially purified peripheral blood monocytes from patients with defects of immune function.
Research Organization:
Laboratory of Immune Effector Function, Howard Hughes Medical Institute, Durham, NC
OSTI ID:
6965659
Journal Information:
Blood; (United States), Journal Name: Blood; (United States) Vol. 63:3; ISSN BLOOA
Country of Publication:
United States
Language:
English

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Related Subjects

550201 -- Biochemistry-- Tracer Techniques
550301* -- Cytology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
DAYS LIVING RADIOISOTOPES
EVEN-ODD NUCLEI
GUINEA PIGS
HORMONES
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LEUKOCYTES
LIGHT NUCLEI
MAMMALS
MAN
MATERIALS
MONOCYTES
NUCLEI
PEPTIDE HORMONES
PRIMATES
RADIOASSAY
RADIOIMMUNOASSAY
RADIOISOTOPES
REACTION KINETICS
RECEPTORS
RODENTS
SULFUR 35
SULFUR ISOTOPES
TRACER TECHNIQUES
VERTEBRATES