Identification and molecular cloning of tactile: A novel human T cell activation antigen that is a member of the Ig gene superfamily
- Stanford Univ. School of Medicine, Stanford, CA (United States)
- Stanford Univ. Medical Center, Stanford, CA (United States) Stanford Univ. School of Medicine, Stanford, CA (United States)
The authors have identified and cloned cDNA for a novel cell-surface protein named Tactile for T cell activation, increased late expression. It is expressed on normal T cell lines and clones, and some transformed T cells, but no other cultured cell lines tested. It is expressed at low levels on peripheral T cells and is strongly up-regulated after activation, peaking 6 to 9 days after the activating stimulus. It is also up-regulated on NK cells activated in allogeneic cultures. It is not found on peripheral B cells but is expressed at very low levels on activated B cells. Tactile-specific mAb immuno-precipitates a band of 160 kDa when reduced and bands of 240, 180, and 160 kDa nonreduced. Using an antiserum produced with affinity-purified Tactile protein to screen a [lambda]gt11 library, the authors have identified Tactile cDNA. Northern blot analysis shows an expression pattern similar to that of the protein and transfection of COS cells with the full-length 5.2-kb cDNA results in cell-surface expression. Comparison with the sequence databanks show that Tactile is a member of the immunoglobulin gene superfamily, with similarity to Drosophila amalgam, the melanoma Ag MUC-18, members of the carcinoembryonic Ag family, the poliovirus receptor, and the neural cell adhesion molecule. The deduced primary sequence encodes a protein with three Ig domains, a long serine/threonine/proline-rich region typical of an extensively O-glycosylated domain, a transmembrane domain, and a 45 residue cytoplasmic domain. These data suggest that Tactile may be involved in adhesive interactions of activated T and NK cells during the late phase of the immune response.
- OSTI ID:
- 6965136
- Journal Information:
- Journal of Immunology; (United States), Vol. 148:8; ISSN 0022-1767
- Country of Publication:
- United States
- Language:
- English
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ANTIGENS
GENES
DETECTION
IMMUNOGLOBULINS
LYMPHOCYTES
METABOLIC ACTIVATION
MEMBRANE PROTEINS
DNA-CLONING
ANIMAL CELLS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CLONING
CONNECTIVE TISSUE CELLS
DNA HYBRIDIZATION
GLOBULINS
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550400* - Genetics