Expression cloning and chromosomal mapping of the leukocyte activation antigen CD97, a new seven-span transmembrane molecule of the secretin receptor superfamily with an unusual extracellular domain
Journal Article
·
· Journal of Immunology
OSTI ID:160155
- Univ. of Amsterdam (Netherlands)
- Univ. of Amsterdam (Netherlands); and others
CD97 is a monomeric glycoprotein of 75 to 85 kDa that is induced rapidly on the surface of most leukocytes upon activation. We herein report the isolation of a cDNA encoding human CD97 by expression cloning in COS cells. The 3-kb cDNA clone encodes a mature polypeptide chain of 722 amino acids with a predicted molecular mass of 79 kDa. Within the C-terminal part of the protein, a region with seven hydrophobic segments was identified, suggesting that CD97 is a seven-span transmembrane molecule. Sequence comparison indicates that CD97 is the first leukocyte Ag in a recently described superfamily that includes the receptors for secretin, calcitonin, and other mammalian and insect peptide hormones. Different from these receptors, CD97 has an extended extracellular region of 433 amino acids that possesses three N-terminal epidermal growth factor-like domains, two of them with a calcium-binding site, and single Arg-Gly-Asp (RGD) motif. The existence of structural elements characteristic for extracellular matrix proteins in a seven-span transmembrane molecule makes CD97 a receptor potentially involved in both adhesion and signaling processes early after leukocyte activation. The gene encoding CD97 is localized on chromosome 19 (19p13.12-13.2).
- OSTI ID:
- 160155
- Journal Information:
- Journal of Immunology, Journal Name: Journal of Immunology Journal Issue: 4 Vol. 155; ISSN JOIMA3; ISSN 0022-1767
- Country of Publication:
- United States
- Language:
- English
Similar Records
EMR1, an unusual member in the family of hormone receptors with seven transmembrane segments
cDNA for the human. beta. /sub 2/-adrenergic receptor: a protein with multiple membrane-spanning domains and encoded by a gene whose chromosomal location is shared with that of the receptor for platelet-derived growth factor
Localization of the gene encoding the secretin receptor, SCTR, on human chromosome 2q14.1 by fluorescence in situ hybridization and chromosome morphometry
Journal Article
·
Sun Mar 19 23:00:00 EST 1995
· Genomics
·
OSTI ID:219897
cDNA for the human. beta. /sub 2/-adrenergic receptor: a protein with multiple membrane-spanning domains and encoded by a gene whose chromosomal location is shared with that of the receptor for platelet-derived growth factor
Journal Article
·
Wed Dec 31 19:00:00 EST 1986
· Proc. Natl. Acad. Sci. U.S.A.; (United States)
·
OSTI ID:5725686
Localization of the gene encoding the secretin receptor, SCTR, on human chromosome 2q14.1 by fluorescence in situ hybridization and chromosome morphometry
Journal Article
·
Tue Oct 10 00:00:00 EDT 1995
· Genomics
·
OSTI ID:443895