Nitric oxide mediates glutamate-linked enhancement of cGMP levels in the cerebellum

Journal Article · Wed Nov 01 04:00:00 EST 1989 · Proceedings of the National Academy of Sciences of the United States of America; (USA)

DOI:https://doi.org/10.1073/pnas.86.22.9030· OSTI ID:6957883

Bredt, D S; Snyder, S H ^[1]

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1. Johns Hopkins Univ. School of Medicine, Baltimore (USA)

Nitric oxide, which mediates influences of numerous neurotransmitters and modulators on vascular smooth muscle and leukocytes, can be formed in the brain from arginine by an enzymatic activity that stoichiometrically generates citrulline. The authors show that glutamate and related amino acids, such as N-methyl-D-aspartate, markedly stimulate arginine-citrulline transformation in cerebellar slices stoichiometrically with enhancement of cGMP levels. N{sup {omega}}-monomethyl-L-arginine blocks the augmentation both of citrulline and cGMP with identical potencies. Arginine competitively reverses both effects of N{sup {omega}}-monomethyl-L-arginine with the same potencies. Hemoglobin, which complexes nitric oxide, prevents the stimulation by N-methyl-D-aspartate of cGMP levels, and superoxide dismutase, which elevates nitric oxide levels, increases cGMP formation. These data establish that nitric oxide mediates the stimulation by glutamate of cGMP formation.

• OSTI ID: 6957883
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (USA), Journal Name: Proceedings of the National Academy of Sciences of the United States of America; (USA) Vol. 86:22; ISSN PNASA; ISSN 0027-8424
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
ARGININE
AROMATICS
ASPARTIC ACID
AUTONOMIC NERVOUS SYSTEM AGENTS
AZAARENES
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BIOSYNTHESIS
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
BRAIN
CARBOXYLIC ACIDS
CENTRAL NERVOUS SYSTEM
CEREBELLUM
CHALCOGENIDES
CITRULLINE
DOSE-RESPONSE RELATIONSHIPS
DRUGS
ENZYMES
GLOBINS
GLUTAMIC ACID
GUANOSINE
HEMOGLOBIN
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
HYDROGEN COMPOUNDS
LEUKOCYTES
MATERIALS
MUSCLES
NERVOUS SYSTEM
NEUROREGULATORS
NITRIC OXIDE
NITROGEN COMPOUNDS
NITROGEN OXIDES
NUCLEOSIDES
NUCLEOTIDES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
OXIDES
OXIDOREDUCTASES
OXYGEN COMPOUNDS
PIGMENTS
PORPHYRINS
PROTEINS
PURINES
RIBOSIDES
STOICHIOMETRY
SUPEROXIDE DISMUTASE
SYNTHESIS
TRITIUM COMPOUNDS