Evidence for evoked release of adenosine and glutamate from cultured cerebellar granule cells
Journal Article
·
· Neurochemical Research; (USA)
- Univ. of Copenhagen (Denmark)
Evoked release of ({sup 3}H)-D-aspartate which labels the neurotransmitter glutamate pool in cultured cerebellar granule cells was compared with evoked release of adenosine from similar cultures. It was found that both adenosine and (3H)-D-aspartate could be released from the neurons in a calcium dependent manner after depolarization of the cells with either 10-100 microM glutamate or 50 mM KCl. Cultures of cerebellar granule cells treated with 50 microM kainate to eliminate GABAergic neurons behaved in the same way. This together with the observation that cultured astrocytes did not exhibit a calcium dependent, potassium stimulated adenosine release strongly suggest that cerebellar granule cells release adenosine in a neurotransmitter-like fashion together with glutamate which is the classical neurotransmitter of these neurons. Studies of the metabolism of adenosine showed that in the granule cells adenosine is rapidly metabolized to ATP, ADP, and AMP, but in spite of this, adenosine was found to be released preferential to ATP.
- OSTI ID:
- 5036219
- Journal Information:
- Neurochemical Research; (USA), Journal Name: Neurochemical Research; (USA) Vol. 14:9; ISSN 0364-3190; ISSN NERED
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550201* -- Biochemistry-- Tracer Techniques
550501 -- Metabolism-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ADENOSINE
ADP
ALKALI METAL COMPOUNDS
ALKALINE EARTH METALS
AMINO ACIDS
AMP
ANIMAL CELLS
ANIMALS
ASPARTIC ACID
ATP
BIOELECTRICITY
BODY
BRAIN
CALCIUM
CARBOXYLIC ACIDS
CELL CULTURES
CENTRAL NERVOUS SYSTEM
CEREBELLUM
CHLORIDES
CHLORINE COMPOUNDS
ELECTRICITY
ELEMENTS
GLUTAMIC ACID
HALIDES
HALOGEN COMPOUNDS
HYDROGEN COMPOUNDS
ISOTOPE APPLICATIONS
MAMMALS
METABOLISM
METALS
MICE
NERVE CELLS
NERVOUS SYSTEM
NUCLEOSIDES
NUCLEOTIDES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
POTASSIUM CHLORIDES
POTASSIUM COMPOUNDS
RIBOSIDES
RODENTS
SECRETION
SOMATIC CELLS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VERTEBRATES
550501 -- Metabolism-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ADENOSINE
ADP
ALKALI METAL COMPOUNDS
ALKALINE EARTH METALS
AMINO ACIDS
AMP
ANIMAL CELLS
ANIMALS
ASPARTIC ACID
ATP
BIOELECTRICITY
BODY
BRAIN
CALCIUM
CARBOXYLIC ACIDS
CELL CULTURES
CENTRAL NERVOUS SYSTEM
CEREBELLUM
CHLORIDES
CHLORINE COMPOUNDS
ELECTRICITY
ELEMENTS
GLUTAMIC ACID
HALIDES
HALOGEN COMPOUNDS
HYDROGEN COMPOUNDS
ISOTOPE APPLICATIONS
MAMMALS
METABOLISM
METALS
MICE
NERVE CELLS
NERVOUS SYSTEM
NUCLEOSIDES
NUCLEOTIDES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
POTASSIUM CHLORIDES
POTASSIUM COMPOUNDS
RIBOSIDES
RODENTS
SECRETION
SOMATIC CELLS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VERTEBRATES