The loss of plasma membrane lysopip and an increase of PIP sub 2 result from treatment of carrot cells with fungal enzymes

Journal Article · Sat Apr 01 00:00:00 EST 1989 · Plant Physiology, Supplement; (USA)

OSTI ID:6957463

Chen, Q; Boss, W F ^[1]

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1. North Carolina State Univ., Raleigh (USA)

The plasma membranes of carrot cells grown in suspension culture are enriched with PIP, lysoPIP, and PIP{sub 2}. To determine whether or not these lipids are involved in signal transduction, we have challenged the cells with a mixture of fungal cellulases, Driselase, and monitored the changes in the phosphoinositides and in the phosphoinositide kinase activity. With cell prelabeled with ({sup 3}H)inositol, two major changes are observed: (1) lysoPIP decreases 30% compared to the sorbitol control and (2) PIP{sub 2} doubles. There is no increase in IP, IP{sub 2}, or IP{sub 3}. In vitro phosphorylation studies using ({gamma}-{sup 32}P)ATP indicate that the increase in PIP{sub 2} is due in part to activation of the PIP kinase. These data suggest that the role of the polyphosphoinositides in signal transduction in plants may involve activation of the PIP kinase and/or activation of A type phospholipases rather than C type phospholipases.

• OSTI ID: 6957463
• Journal Information: Plant Physiology, Supplement; (USA), Vol. 89:4; ISSN 0079-2241
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
PLANT CELLS
PHYSIOLOGY
CARROTS
CELL MEMBRANES
INOSITOL
LIPASES
LIPIDS
PHOSPHORUS 32
PHOSPHORYLATION
PHOSPHOTRANSFERASES
TRACER TECHNIQUES
TRITIUM COMPOUNDS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CARBOHYDRATES
CARBOXYLESTERASES
CELL CONSTITUENTS
CHEMICAL REACTIONS
DAYS LIVING RADIOISOTOPES
ENZYMES
ESTERASES
FOOD
HYDROGEN COMPOUNDS
HYDROLASES
INOSITOLS
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
MAGNOLIOPHYTA
MAGNOLIOPSIDA
MEMBRANES
MONOSACCHARIDES
NUCLEI
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PHOSPHORUS ISOTOPES
PHOSPHORUS-GROUP TRANSFERASES
PLANTS
RADIOISOTOPES
SACCHARIDES
TRANSFERASES
VEGETABLES
551001* - Physiological Systems- Tracer Techniques