Characterization of D/sub 1/ dopamine receptors in the central nervous system
Several lines of evidence suggest an association of central nervous system dopaminergic systems in the etiology of the schizophrenia. Interest in the role of D/sub 1/ dopamine receptors has revived with the advent of selective drugs for this dopamine receptor, particularly the D/sub 1/ dopamine receptor antagonists, SCH23390. (/sup 3/H)SCH23390 represents a superior radioligand for labeling the two-state striatal D/sub 1/ dopamine receptor in that its high percent specific binding makes it especially suitable for detailed mechanistic studies of this receptor. Striatal D/sub 1/ dopamine receptors have been shown to mediate the stimulation of adenylate cyclase activity via a guanine nucleotide regulatory subunit. Forskolin acts in a synergistic manner with dopamine agonists, guanine nucleotides or sodium fluoride to potentiate the stimulation of rat striatal adenylate cyclase activity mediated by these reagents. By using the aforementioned reagents and the irreversible receptor modifying reagent N-ethoxycarbonyl-2-ethoxy-1,2,-dihydroquinoline, we demonstrated that the D/sub 1/ dopamine receptor population in rat striatum is not a stoichiometrically-limiting factor in agonist stimulation of adenylate cyclase activity.
- Research Organization:
- California Univ., San Diego (USA)
- OSTI ID:
- 6956708
- Resource Relation:
- Other Information: Thesis (Ph. D.)
- Country of Publication:
- United States
- Language:
- English
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550201* - Biochemistry- Tracer Techniques
550901 - Pathology- Tracer Techniques