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Dopamine inhibition of anterior pituitary adenylate cyclase is mediated through the high-affinity state of the D/sub 2/ receptor

Journal Article · · Life Sci.; (United States)
;

The diterpinoid forskolin stimulated adenylate cyclase activity (measured by conversion of (/sup 3/H)-ATP to (/sup 3/H)-cAMP) in anterior pituitary from male and female rats. Inhibition of stimulated adenylate cyclase activity by potent dopaminergic agonists was demonstrable only in female anterior pituitary. The inhibition of adenylate cyclase activity displayed a typically dopaminergic rank order of agonist potencies and could be completely reversed by a specific dopamine receptor antagonist. The IC/sub 50/ values of dopamine agonist inhibition of adenylate cyclase activity correlated with equal molarity with the dissociation constant of the high-affinity dopamine agonist-detected receptor binding site and with the IC/sub 50/ values for inhibition of prolactin secretion. These findings support the hypothesis that it is the high-affinity form of the D/sub 2/ dopamine receptor in anterior pituitary which is responsible for mediating the dopaminergic function of attenuating adenylate cyclase activity. 12 references, 4 figures, 1 table.

Research Organization:
Univ. of Toronto, Ontario
OSTI ID:
5569574
Journal Information:
Life Sci.; (United States), Journal Name: Life Sci.; (United States) Vol. 37:4; ISSN LIFSA
Country of Publication:
United States
Language:
English

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Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AFFINITY
AMINES
AMP
ANIMALS
AROMATICS
ATP
AUTONOMIC NERVOUS SYSTEM AGENTS
BIOLOGICAL EFFECTS
BODY
CARDIOTONICS
CARDIOVASCULAR AGENTS
CHEMICAL BONDS
CYCLASES
DOPAMINE
DRUGS
ENDOCRINE GLANDS
ENZYME ACTIVITY
ENZYMES
GLANDS
GONADOTROPINS
HORMONES
HYDROXY COMPOUNDS
INHIBITION
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LTH
LYASES
MAMMALS
NEUROREGULATORS
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANS
PEPTIDE HORMONES
PHENOLS
PITUITARY GLAND
PITUITARY HORMONES
POLYPHENOLS
RATS
RECEPTORS
RODENTS
SEX DEPENDENCE
SYMPATHOMIMETICS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VERTEBRATES