Effect of CO sub 2 concentration on protein biosynthesis and carbonic anhydrase expression in Chlamydomonas reinhardtii

Journal Article · Mon Aug 01 04:00:00 EDT 1988 · Plant Physiology; (USA)

DOI:https://doi.org/10.1104/pp.87.4.833· OSTI ID:6956601

Bailly, J; Coleman, J R ^[1]

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1. Univ. of Toronto, Ontario (Canada)

The effect of external inorganic carbon (C{sub i}) concentrations on protein biosynthesis and carbonic anhydrase (CA) mRNA abundance were examined in the eukaryotic alga Chlamydomonas reinhardtii. Transfer of high CO{sub 2} (5%) grown algae to air levels of CO{sub 2} resulted in the transitory synthesis of two polypeptides of approximately 49,000 and 52,000 daltons as well as prolonged synthesis and accumulation of the 37,000 dalton CA monomer and an unidentified 20,000 dalton polypeptide. The gene coding for carbonic anhydrase was isolated from a genomic expression library and subjected to restriction endonuclease analysis. Southern blot analysis of chromosomal DNA indicates that only a single copy of the gene is present. The 2.5 kilobase DNA fragment hybridizes specially to a 1.4 kilobase transcript in RNA isolated from air-grown cells and from cells grown on 5% CO{sub 2} that have been exposed to air levels of CO{sub 2}. Maximum mRNA abundance was observed after 1 to 3 hours of exposure to air. Transfer of air-grown cells to a high CO{sub 2} environment resulted in the elimination of the CA transcript after 60 minutes of exposure. Changes in CA transcript abundance in response to external C{sub i} concentrations occurred in the presence or absence of light.

• OSTI ID: 6956601
• Journal Information: Plant Physiology; (USA), Journal Name: Plant Physiology; (USA) Vol. 87:4; ISSN PLPHA; ISSN 0032-0889
• Country of Publication: United States
• Language: English

Related Subjects

550200 -- Biochemistry
560300* -- Chemicals Metabolism & Toxicology
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALGAE
BIOLOGICAL ADAPTATION
BIOLOGICAL EFFECTS
BIOSYNTHESIS
CARBON
CARBON COMPOUNDS
CARBON DIOXIDE
CARBON OXIDES
CARBON-OXYGEN LYASES
CARBONIC ANHYDRASE
CHALCOGENIDES
CHLAMYDOMONAS
CHLOROPHYCOTA
ELEMENTS
ENZYMES
GENES
HYDRO-LYASES
LYASES
MESSENGER-RNA
MICROORGANISMS
NONMETALS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
OXIDES
OXYGEN COMPOUNDS
PHYSIOLOGY
PLANTS
PROTEINS
RNA
SYNTHESIS
UNICELLULAR ALGAE