Light-induced carbonic anhydrase expression in Chlamydomonas reinhardtii

Journal Article · Thu Nov 01 04:00:00 EST 1990 · Plant Physiology; (USA)

DOI:https://doi.org/10.1104/pp.94.3.1103· OSTI ID:5841848

Dionisio-Sese, M L; Fukuzawa, Hideya; Miyachi, Shigetoh ^[1]

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1. Univ. of Tokyo (Japan)

The effect of external inorganic carbon concentration and light on carbonic anhydrase (CA) protein accumulation and steady-state mRNA levels were examined in Chlamydomonas reinhardtii. When photoautotrophically grown high-CO{sub 2} cells were transferred to low-CO{sub 2} conditions, they exhibited a significant accumulation of the 2.0-kilobase CA transcript after 1 hour with the maximum level reached after 2 hours. An increase in the accumulation of the 37-kilodalton CA monomer was observed after 2-hour exposure to air. Cells allowed to adapt to air levels of CO{sub 2} in the dark showed neither an increase in CA mRNA abundance nor in the accumulation of the enzyme. Similarly, addition of 10 micromole 3-(3,4-dichlorophenyl)-1,1-dimethylurea immediately after transferring high-CO{sub 2} cells to low-CO{sub 2} condition did not cause an increase in CA transcript abundance and enzyme accumulation, suggesting that photosynthesis is absolutely required in the regulation of CA transcript abundance. In addition to the photosynthesis-dependent process, a blue light stimulated mechanism is also involved in CA transcript regulation. Experiments with transcription inhibitors confirmed the notion that the gene for carbonic anhydrase enzyme is encoded by the nuclear genome and that the induction of the enzyme depends heavily on the transcription of the CA gene.

• OSTI ID: 5841848
• Journal Information: Plant Physiology; (USA), Journal Name: Plant Physiology; (USA) Vol. 94:3; ISSN 0032-0889; ISSN PLPHA
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
ALGAE
BIOLOGICAL EFFECTS
CARBON
CARBON COMPOUNDS
CARBON DIOXIDE
CARBON OXIDES
CARBON-OXYGEN LYASES
CHALCOGENIDES
CHEMICAL REACTIONS
CHLAMYDOMONAS
CHLOROPHYCOTA
ELECTROMAGNETIC RADIATION
ELEMENTS
ENZYME ACTIVITY
ENZYMES
GENES
HYDRO-LYASES
LYASES
MICROORGANISMS
NONMETALS
OXIDES
OXYGEN COMPOUNDS
PHOTOCHEMICAL REACTIONS
PHOTOSYNTHESIS
PLANTS
RADIATIONS
SYNTHESIS
TRANSCRIPTION
UNICELLULAR ALGAE
VISIBLE RADIATION