Interspecies cytogenetic comparisons: studies with x-radiation and bleomycin sulfate

Technical Report · Wed Jan 01 04:00:00 EST 1992

OSTI ID:6952946

Kligerman, A D; Bryant, M F; Doerr, C L; Halperin, E C; Kwanyuen, P

A series of in vitro experiments were conducted to determine if there are innate differences in the sensitivity of peripheral blood lymphocytes (PBLs) from different mammalian species to clastogens. Mouse, rat, and human whole blood samples were exposed to either 0, 0.38, 0.75, 1.5, or 3.0 Gy x-radiation or 0, 5, 10, 20, 40, or 80 micrograms/ml bleomycin for 4 h. Bromodeoxyuridine-containing cultures were initiated and the PBLs stimulated to divide with phytohemagglutinin. All cultures were harvested following a 3-h colcemid treatment. Slides were made and differentially stained, and first-division metaphases were scored for chromosome aberrations. In the x-radiation studies human PBLs were significantly more sensitive than mouse PBLs which were in turn more sensitive than rat PBLs as measured by either the total percent aberrant cells or the number of dicentrics. Results with bleomycin suggest that the mouse and human PBLs are equally sensitive to the clastogenic effects of bleomycin. These results indicate that there may be inherent differences in sensitivity among PBLs of mammalian species; however, more studies are needed to determine if the differences presented here hold for other agents.

• Research Organization: Environmental Health Research and Testing, Inc., Research Triangle Park, NC (United States)
• OSTI ID: 6952946
• Report Number(s): PB-92-227438/XAB; CNN: EPA-68-02-4456
• Country of Publication: United States
• Language: English

Related Subjects

560120* -- Radiation Effects on Biochemicals
Cells
& Tissue Culture
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AGGLUTININS
ANIMAL CELLS
ANIMALS
ANTI-INFECTIVE AGENTS
ANTIBIOTICS
ANTIBODIES
ANTIMETABOLITES
ANTIMITOTIC DRUGS
ANTINEOPLASTIC DRUGS
AZINES
BIOLOGICAL MATERIALS
BIOLOGICAL VARIABILITY
BLEOMYCIN
BLOOD
BLOOD CELLS
BODY FLUIDS
BROMOURACILS
BUDR
CARBOHYDRATES
CONNECTIVE TISSUE CELLS
DOSE-RESPONSE RELATIONSHIPS
DRUGS
ELECTROMAGNETIC RADIATION
GENETIC VARIABILITY
HEMAGGLUTININS
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
IONIZING RADIATIONS
LEUKOCYTES
LYMPHOCYTES
MAMMALS
MAN
MATERIALS
MICE
MITOGENS
MUCOPROTEINS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC BROMINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PHYTOHEMAGGLUTININ
POLYSACCHARIDES
PRIMATES
PROTEINS
PYRIMIDINES
RADIATIONS
RADIOSENSITIVITY
RATS
RIBOSIDES
RODENTS
SACCHARIDES
SOMATIC CELLS
URACILS
VERTEBRATES
X RADIATION