Induction of micronuclei by x radiation in human, mouse, and rat peripheral blood lymphocytes

Technical Report · Tue Jan 01 04:00:00 EST 1991

OSTI ID:6098529

Erexson, G L; Kligerman, A D; Bryant, M F; Sontag, M R; Halperin, E C

The study compared the radiosensitivity of human, rat and mouse peripheral blood lymphocytes (PBLs) by analyzing micronuclei (MN) in cytochalasin B-induced binucleated (BN) cells. For each species and dose 4-ml aliquots of whole blood were X-irradiated to obtain doses of 38, 75, 150 or 300 cGy. Controls were sham-irradiated. After exposure to X-rays, mononuclear leukocytes were isolated using density gradients and cultured in RPMI 1640 medium containing phytohemagglutinin to stimulate mitogenesis. At 21 h cytochalasin B was added to produce BN PBLs, and all cultures were harvested at 52 h post-initiation using a cytocentrifuge. Significant dose-dependent increases in the percentage of micronucleated cells and the number of MN per BN cell were observed in all three species. The linear-quadratic regression curves for the total percentage of micronucleated cells for the three species were similar; however, the curve for the mouse PBLs had a larger quadratic component than either of the curves for the rat or human PBLs. Although the correlation between the percentage of cells with MN and those with chromosome aberrations was high (r squared > 0.95), the mouse and rat PBLs were over twice as efficient as human PBLs in forming MN from presumed acentric fragments. These data indicate that the induction of MN in BN cells following ionizing radiation is similar in human, rat and mouse PBLs, but care must be taken in using the MN results to predict frequencies of cells with chromosomal aberrations.

• Research Organization: Environmental Health Research and Testing, Inc., Research Triangle Park, NC (United States)
• OSTI ID: 6098529
• Report Number(s): PB-92-113315/XAB; CNN: EPA-68-02-4456
• Country of Publication: United States
• Language: English

Related Subjects

560120* -- Radiation Effects on Biochemicals
Cells
& Tissue Culture
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AGGLUTININS
ANIMAL CELLS
ANIMALS
ANTIBODIES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARBOHYDRATES
CELL CONSTITUENTS
CELL NUCLEI
CONNECTIVE TISSUE CELLS
DOSE-RESPONSE RELATIONSHIPS
DOSES
ELECTROMAGNETIC RADIATION
HEMAGGLUTININS
IONIZING RADIATIONS
LEUKOCYTES
LYMPHOCYTES
MAMMALS
MAN
MATERIALS
MICE
MITOGENS
MUCOPROTEINS
MUTATION FREQUENCY
ORGANIC COMPOUNDS
PHYTOHEMAGGLUTININ
POLYSACCHARIDES
PRIMATES
PROTEINS
RADIATION DOSES
RADIATIONS
RADIOINDUCTION
RADIOSENSITIVITY
RATS
REGIONAL ANALYSIS
RODENTS
SACCHARIDES
SOMATIC CELLS
VERTEBRATES
X RADIATION