Genetic recombination and complementation between bacteriophage T7 and cloned fragments of T7 DNA

Journal Article · Mon May 01 00:00:00 EDT 1978 · Proc. Natl. Acad. Sci. U.S.A.; (United States)

DOI:https://doi.org/10.1073/pnas.75.5.2276· OSTI ID:6952452

Campbell, J L; Richardson, C C; Studier, F W

Fragments of phage T7 DNA have been cloned in Escherichia coli by using the plasmid pMB9. Such cloned fragments are able to recombine with infecting phages, thus providing a means to integrate the physical and genetic maps of T7 DNA. Approximately 65% of the T7 DNA molecule has been found in clones so far, and analysis of these clones has mapped genes 12-17 with an accuracy of about 1% the total length of T7 DNA. At least some cloned segments can supply T7 functions to infecting phages.

• Research Organization: Harvard Medical School, Boston, MA (United States)
• OSTI ID: 6952452
• Journal Information: Proc. Natl. Acad. Sci. U.S.A.; (United States), Vol. 75:5
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
BACTERIOPHAGES
GENE RECOMBINATION
DNA
CLONE CELLS
ESCHERICHIA COLI
GENETIC MAPPING
PLASMIDS
BACTERIA
CELL CONSTITUENTS
CELL CULTURES
MICROORGANISMS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PARASITES
VIRUSES
550400* - Genetics