In vitro recombination of bacteriophage T7 DNA damaged by uv radiation
Journal Article
·
· J. Virol.; (United States)
OSTI ID:5369384
A system capable of in vitro packaging of exogenous bacteriophage T7 DNA has been used to monitor the biological activity of DNA replicated in vitro. This system has been used to follow the effects of uv radiation on in vitro replication and recombination. During the in vitro replication process, a considerable exchange of genetic information occurs between T7 DNA molecules present in the reaction mixture. This in vitro recombination is reflected in the genotype of the T7 phage produced after in vitro encapsulation; depending on the genetic markers selected, recombinants can comprise nearly 20% of the total phage production. When uv-irradiated DNA is incubated in this system, the amount of in vitro synthesis is reduced and the total amount of viable phage produced after in vitro packaging is diminished. In vitro recombination rates are also lower when the participating DNA molecules have been exposed to uv. However, biochemical and genetic measurements confirmed that there is little or no transfer of pyrimidine dimers from irradiated DNA into undamaged molecules.
- Research Organization:
- Oak Ridge National Lab., TN
- DOE Contract Number:
- W-7405-ENG-26
- OSTI ID:
- 5369384
- Journal Information:
- J. Virol.; (United States), Journal Name: J. Virol.; (United States) Vol. 33:1; ISSN JOVIA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550200 -- Biochemistry
550700 -- Microbiology
560112 -- Radiation Effects on Biochemicals-- In Microorganisms-- (-1987)
560131* -- Radiation Effects on Microorganisms-- Basic Studies-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AZINES
BACTERIOPHAGES
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOSYNTHESIS
DIMERS
DNA
DNA REPLICATION
ELECTROMAGNETIC RADIATION
GENE RECOMBINATION
HETEROCYCLIC COMPOUNDS
IN VITRO
MICROORGANISMS
NUCLEIC ACID REPLICATION
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PARASITES
PYRIMIDINES
RADIATION EFFECTS
RADIATIONS
SYNTHESIS
ULTRAVIOLET RADIATION
VIRUSES
550700 -- Microbiology
560112 -- Radiation Effects on Biochemicals-- In Microorganisms-- (-1987)
560131* -- Radiation Effects on Microorganisms-- Basic Studies-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AZINES
BACTERIOPHAGES
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOSYNTHESIS
DIMERS
DNA
DNA REPLICATION
ELECTROMAGNETIC RADIATION
GENE RECOMBINATION
HETEROCYCLIC COMPOUNDS
IN VITRO
MICROORGANISMS
NUCLEIC ACID REPLICATION
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PARASITES
PYRIMIDINES
RADIATION EFFECTS
RADIATIONS
SYNTHESIS
ULTRAVIOLET RADIATION
VIRUSES