Inhibitors of poly (ADP-ribose) synthesis inhibit the two types of repair of potentially lethal damage

Journal Article · Wed Jun 15 04:00:00 EDT 1994 · International Journal of Radiation Oncology, Biology and Physics; (United States)

DOI:https://doi.org/10.1016/0360-3016(94)90459-6· OSTI ID:6947148

Utsumi, Hiroshi ^[1]; Elkind, M M ^[2]

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1. Kyoto Univ., Osaka (Japan)
2. Colorado State Univ., Ft. Collins, CO (United States)

The purpose of this study was to examine whether 3-amino-benzamide (3ABA), an inhibitor of poly (ADP-ribose) synthesis, inhibits the two types of potentially lethal damage (PLD) repair, termed slow and fast. The fast-type PLD repair was measured by the decrease in survival of V79 Chinese hamster cells by postirradiation treatment with 3ABA. The slow-type PLD repair was measured by the increase in survival by posttreatment with conditioned medium (CM), which became conditioned by growing a crowed culture of cells and supports the slow-type PLD repair. Up to 1 mM 3-ABA inhibited the slow type repair; at doses of 2 mM and above, it inhibited the fast type of PLD repair. There are quantitative differences in cellular effects of 3ABA dependent on concentration. Poly (ADP-ribose) appears to play an important role in the PLD repairs and has little effect on the repair of sublethal damage. 10 refs., 2 figs.

• OSTI ID: 6947148
• Journal Information: International Journal of Radiation Oncology, Biology and Physics; (United States), Journal Name: International Journal of Radiation Oncology, Biology and Physics; (United States) Vol. 29:3; ISSN IOBPD3; ISSN 0360-3016
• Country of Publication: United States
• Language: English

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