Inhibitors of poly(ADP-ribose) synthesis enhance X-ray killing of log-phase Chinese hamster cells

Journal Article · Thu Mar 01 04:00:00 EST 1984 · Radiat. Res.; (United States)

DOI:https://doi.org/10.2307/3576146· OSTI ID:6915273

Ben-Hur, E; Utsumi, H; Elkind, M M

Postirradiation incubation of V79 Chinese hamster cells with inhibitors of poly(ADP-ribose) synthesis was found to potentiate the killing of cells by X rays. Potentiation increased with incubation time and with concentration of the inhibitor. Preirradiation incubation had only a small effect. The enhanced response correlated well with the known extent of the inhibition of poly(ADP-ribose) synthesis. A radiation-sensitive line, V79-AL162/S-10, was affected to a lesser extent than the normal cells. Cells repaired the radiation damage with which the inhibitors interacted within 1 hr, a process that has similar kinetics to what is observed when a postirradiation treatment with hypertonic buffer is used. However, the sectors of damage affected by inhibitors of poly(ADP-ribose) synthesis and hypertonic buffer do not entirely overlap. The inhibitor nicotinamide enhanced the killing mainly of late S-phase cells and did not affect cells at the G/sub 1//S border. It is concluded that the repair process(es) involving poly(ADP-ribose) synthesis is important for cell survival in repair-competent cells and that the radiation-sensitive cells that were examined are partially deficient in a repair pathway in which poly(ADP-ribose) participates.

• Research Organization: Colorado State Univ., Fort Collins
• OSTI ID: 6915273
• Journal Information: Radiat. Res.; (United States), Journal Name: Radiat. Res.; (United States) Vol. 97:3; ISSN RAREA
• Country of Publication: United States
• Language: English

Related Subjects

550300 -- Cytology
560121* -- Radiation Effects on Cells-- External Source-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AMIDES
ANIMAL CELLS
ANIMALS
AZINES
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
BIOSYNTHESIS
CELL KILLING
CONNECTIVE TISSUE CELLS
ELECTROMAGNETIC RADIATION
ENZYME INHIBITORS
ENZYMES
FIBROBLASTS
FRACTIONATED IRRADIATION
HAMSTERS
HETEROCYCLIC COMPOUNDS
IONIZING RADIATIONS
IRRADIATION
MAMMALS
NICOTINAMIDE
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PHOSPHORUS-GROUP TRANSFERASES
PYRIDINES
RADIATIONS
RADIOINDUCTION
RADIOSENSITIVITY EFFECTS
RECOVERY
REPAIR
RODENTS
SOMATIC CELLS
SURVIVAL CURVES
SYNTHESIS
TRANSFERASES
VERTEBRATES
VITAMIN B GROUP
VITAMINS
X RADIATION