Apurinic/apyrimidinic endonucleases in repair of pyrimidine dimers and other lesions in DNA
The characteristics of the nicks (single-strand breaks) introduced into damaged DNA by Escherichia coli endonucleases III, IV and VI and by phage T4 uv endonuclease have been investigated with E. coli DNA polymerase I. Nicks introduced into depurinated DNA by endonuclease IV or VI provide good primer termini for the polymerase, whereas nicks introduced into depurinated DNA by endonuclease III or into irradiated DNA by T4 uv endonuclease do not. This result suggests that endonuclease IV nicks depurinated DNA on the 5' side of the apurinic site, as does endonuclease VI, whereas endonuclease III has a differnet incision mechanism. T4 uv endonuclease also possesses apurinic endonuclease activity that generates nicks in depurinated DNA with low priming activity for the polymerase. The priming activity of DNA nicked with endonuclease III or T4 uv endonuclease can be enhanced by an additional incubation with endonuclease VI and, to a lesser extent, by incubation with endonuclease IV. These results indicate that endonuclease III and T4 uv endonuclease generate nicks containing apurinic/apyrimidinic sites at their 3' termini and that such sites are not rapidly excised by the 3' ..-->.. 5' activity of DNA polymerase I. However, endonuclease IV or VI apparently can remove such terminal apurinic/apyrimidinic sites as well as cleave on the 5' side of the unnicked sites.
- Research Organization:
- Univ. of California, Berkeley, CA (United States)
- DOE Contract Number:
- DE-AS03-76-SF34
- OSTI ID:
- 6946390
- Journal Information:
- Proc. Natl. Acad. Sci. U.S.A.; (United States), Vol. 77:8
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
DNA
BIOLOGICAL REPAIR
NUCLEASES
ENZYME ACTIVITY
BIOCHEMISTRY
DIMERS
DNA-ASE
ESCHERICHIA COLI
PYRIMIDINES
AZINES
BACTERIA
BIOLOGICAL RECOVERY
CHEMISTRY
ENZYMES
ESTERASES
HETEROCYCLIC COMPOUNDS
HYDROLASES
MICROORGANISMS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PHOSPHATASES
PHOSPHOTRANSFERASES
RECOVERY
REPAIR
TRANSFERASES
550200* - Biochemistry
550700 - Microbiology