Isolation and characterization of transforming growth factors from human malignant gliomas: possible role of transforming growth factors in the pathogenesis of the gliosarcoma
Thesis/Dissertation
·
OSTI ID:6945395
This study provides the first direct experimental evidence that human malignant gliomas secrete soluble polypeptides with TGF-like activity. The conditioned medium from three well-characterized malignant glioma cell lines promote the growth of NRK indicator cells in soft agar. Following acid extraction and gel filtration, TGF-like activity was recovered from all three cell lines. Active fractions from gel filtration in two of the glioma cell lines (U-343 MG-A and SF-210) were further purified by reverse-phase HPLC. The TGF isolated from U-343 MG-A by HPLC is an acid- and heat-stable protein complex whose activity is destroyed by reducing agents and incubation with monospecific anti-TGF-alpha monoclonal antibodies. The NRK colony stimulating activity of this TGF is potentiated by the addition of TGF-beta. The partially purified U-343 MG-A TGF competes with radiolabeled (/sup 125/I)-ECF for the EGF-receptor on A431 epidermoid carcinoma cells. Finally, a total RNA preparation from U-343- MG-A contains a 4.8 kilobase mRNA for TGF-alpha. Therefore, U-343 MG-A secretes a soluble polypeptide with TGF-alpha-like activity. In contrast, the purified SF-210 malignant glioma cell line secretes an acid- and heat-stable TGF with neither TGF-alpha- nor TGF-beta-like activity.
- Research Organization:
- California Univ., San Francisco (USA)
- OSTI ID:
- 6945395
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550201* -- Biochemistry-- Tracer Techniques
550901 -- Pathology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMALS
BETA DECAY RADIOISOTOPES
BIOLOGICAL FUNCTIONS
CHEMICAL COMPOSITION
CHROMATOGRAPHY
COLONY FORMATION
DAYS LIVING RADIOISOTOPES
DISEASES
ELECTRON CAPTURE RADIOISOTOPES
FUNCTIONS
GLIOMAS
GROWTH FACTORS
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LIQUID COLUMN CHROMATOGRAPHY
MAMMALS
MAN
MITOGENS
NEOPLASMS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
PATHOGENESIS
PEPTIDES
POLYPEPTIDES
PRIMATES
PROTEINS
RADIOISOTOPES
SEPARATION PROCESSES
TRACER TECHNIQUES
TUMOR CELLS
VERTEBRATES
550901 -- Pathology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMALS
BETA DECAY RADIOISOTOPES
BIOLOGICAL FUNCTIONS
CHEMICAL COMPOSITION
CHROMATOGRAPHY
COLONY FORMATION
DAYS LIVING RADIOISOTOPES
DISEASES
ELECTRON CAPTURE RADIOISOTOPES
FUNCTIONS
GLIOMAS
GROWTH FACTORS
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LIQUID COLUMN CHROMATOGRAPHY
MAMMALS
MAN
MITOGENS
NEOPLASMS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
PATHOGENESIS
PEPTIDES
POLYPEPTIDES
PRIMATES
PROTEINS
RADIOISOTOPES
SEPARATION PROCESSES
TRACER TECHNIQUES
TUMOR CELLS
VERTEBRATES