Molecular cloning and expression of an additional epidermal growth factor receptor-related gene
- Oncogen, Seattle, WA (USA)
Epidermal growth factor (EGF), transforming growth factor {alpha} (TGF-{alpha}), and amphiregulin are structurally and functionally related growth regulatory proteins. These secreted polypeptides all bind to the 170-kDa cell-surface EGF receptor, activating its intrinsic kinase activity. However, amphiregulin exhibits different activities than EGF and TGF-{alpha} in a number of biological assays. Amphiregulin only partially competes with EGF for binding EGF receptor, and amphiregulin does not induce anchorage-independent growth of normal rat kidney cells (NRK) in the presence of TGF-{beta}. Amphiregulin also appears to abrogate the stimulatory effect of TGF-{alpha} on the growth of several aggressive epithelial carcinomas that over-express EGF receptor. These findings suggest that amphiregulin may interact with a separate receptor in certain cell types. Here the authors report the cloning of another member of the human EGF receptor (HER) family of receptor tyrosine kinases, which were named HER3/ERRB3. The cDNA was isolated from a human carcinoma cell line, and its 6-kilobase transcript was identified in various human tissues. They have generated peptide-specific antisera that recognizes the 160-kDa HER3 protein when transiently expressed in COS cells. These reagents will allow us to determine whether HER3 binds amphiregulin or other growth regulatory proteins and what role HER3 protein plays in the regulation of cell growth.
- OSTI ID:
- 6249928
- Journal Information:
- Proceedings of the National Academy of Sciences of the United States of America; (USA), Vol. 87:13; ISSN 0027-8424
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
GENES
DNA-CLONING
GROWTH FACTORS
RECEPTORS
MEMBRANE PROTEINS
AMINO ACID SEQUENCE
CARCINOMAS
MAN
PHOSPHOTRANSFERASES
RECOMBINANT DNA
TRANSCRIPTION
TUMOR CELLS
TYROSINE
AMINO ACIDS
ANIMAL CELLS
ANIMALS
CARBOXYLIC ACIDS
CLONING
DISEASES
DNA
DNA HYBRIDIZATION
ENZYMES
HYBRIDIZATION
HYDROXY ACIDS
MAMMALS
MITOGENS
MOLECULAR STRUCTURE
NEOPLASMS
NUCLEIC ACIDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
PHOSPHORUS-GROUP TRANSFERASES
PRIMATES
PROTEINS
TRANSFERASES
VERTEBRATES
550201* - Biochemistry- Tracer Techniques