Proteins that interact with GTP during sporulation of Bacillus subtilis
- Univ. of Illinois, Chicago (USA)
During sporulation of Bacillus subtilis, several proteins were shown to interact with GTP in specific ways. UV light was used to cross-link ({alpha}-{sup 32}P)GTP to proteins in cell extracts at different stages of growth. After electrophoresis, 11 bands of radioactivity were found in vegetative cells, 4 more appeared during sporulation, and only 9 remained in mature spores. Based on the labeling pattern with or without UV light to cross-link either ({alpha}-{sup 32}P)GTP or ({gamma}-{sup 32}P)GTP, 11 bands of radioactivity were apparent guanine nucleotide-binding proteins, and 5 bands appeared to be phosphorylated and/or guanylated. Similar results were found with Bacillus megaterium. Assuming the GTP might be a type of signal for sporulation, it could interact with and regulate proteins by at least three mechanisms.
- OSTI ID:
- 6945276
- Journal Information:
- Journal of Bacteriology; (USA), Journal Name: Journal of Bacteriology; (USA) Vol. 171:6; ISSN JOBAA; ISSN 0021-9193
- Country of Publication:
- United States
- Language:
- English
Similar Records
Different small, acid-soluble proteins of the alpha/beta type have interchangeable roles in the heat and uv (ultraviolet) radiation resistance of Bacillus subtilis spores
A structured model for vegetative growth and sporulation in Bacillus thuringiensis
Related Subjects
59 BASIC BIOLOGICAL SCIENCES
AROMATICS
AZAARENES
BACILLUS
BACILLUS SUBTILIS
BACTERIA
BACTERIAL SPORES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
CHEMICAL REACTIONS
DAYS LIVING RADIOISOTOPES
GUANOSINE
HETEROCYCLIC COMPOUNDS
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LIGHT NUCLEI
MICROORGANISMS
MOLECULAR BIOLOGY
NUCLEI
NUCLEOSIDES
NUCLEOTIDES
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PHOSPHORYLATION
PROTEINS
PURINES
RADIOISOTOPES
REACTION KINETICS
RIBOSIDES
SPORES
TRACER TECHNIQUES