Different small, acid-soluble proteins of the alpha/beta type have interchangeable roles in the heat and uv (ultraviolet) radiation resistance of Bacillus subtilis spores
Spores of Bacillus subtilis strains which carry deletion mutations in one gene (sspA) or two genes (sspA and sspB) which code for major alpha/beta-type small, acid-soluble spore proteins (SASP) are known to be much more sensitive to heat and UV radiation than wild-type spores. This heat- and UV-sensitive phenotype was cured completely or in part by introduction into these mutant strains of (i) one or more copies of the sspA or sspB genes themselves; (ii) multiple copies of the B. subtilis sspD gene, which codes for a minor alpha/beta-type SASP; or (iii) multiple copies of the SASP-C genes, which codes for a major alpha/beta-type SASP of Bacillus megaterium. These findings suggest that alpha-beta-type SASP play interchangeable roles in the heat and UV radiation resistance of bacterial spores.
- Research Organization:
- Connecticut Univ. Health Center, Farmington (USA). Dept. of Biochemistry
- OSTI ID:
- 5467931
- Report Number(s):
- AD-A-187376/9/XAB
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BACILLUS
BACILLUS SUBTILIS
BACTERIA
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
ELECTROMAGNETIC RADIATION
ENERGY
GENES
HEAT
MICROORGANISMS
MUTATIONS
ORGANIC COMPOUNDS
PROTEINS
RADIATION EFFECTS
RADIATIONS
SPORES
ULTRAVIOLET RADIATION