Detection of biological warfare agents using the polymerase chain reaction. Final report, June-August 1991
Technical Report
·
OSTI ID:6943738
The detection of biological warfare agents is an important mission for the U.S. Army. This report explores the feasibility of using the polymerase chain reaction as a means of rapid detection of biological warfare agents. Two levels of detection are proposed. The first level is group specific detection, using primers derived from 16S rDNA sequences, to detect various groups of pathogenic bacteria. The second level is species-specific detection using primers derived from DNA sequences, unique to each pathogenic organism targeted for detection. Specific examples of Vibrio cholerae, Francisella tularensis, Yersinia pestis, Staphylococcus aureus, and Bacillus anthracis are described.
- Research Organization:
- Battelle Columbus Labs., Research Triangle Park, NC (United States)
- OSTI ID:
- 6943738
- Report Number(s):
- AD-A-259391/1/XAB; CNN: DAAL03-86-D-0001
- Country of Publication:
- United States
- Language:
- English
Similar Records
Evaluation of the FilmArray® system for detection of Bacillus anthracis, Francisella tularensis, and Yersinia pestis
Species specific identification of spore-producing microbes using the gene sequence of small acid-soluble spore coat proteins for amplification based diagnostics
Novel Agents for Gram-Negative Biodefense Pathogens (CRADA Final Report)
Journal Article
·
Mon Apr 29 00:00:00 EDT 2013
· Journal of Applied Microbiology, 114(4):992-1000
·
OSTI ID:1079117
Species specific identification of spore-producing microbes using the gene sequence of small acid-soluble spore coat proteins for amplification based diagnostics
Patent
·
Mon Dec 31 23:00:00 EST 2001
·
OSTI ID:874841
Novel Agents for Gram-Negative Biodefense Pathogens (CRADA Final Report)
Technical Report
·
Wed Jan 23 23:00:00 EST 2013
·
OSTI ID:1084458
Related Subjects
45 MILITARY TECHNOLOGY, WEAPONRY, AND NATIONAL DEFENSE
450600 -- Military Technology
Weaponry
& National Defense-- Chemical & Biological-- (1990)
560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BACILLUS
BACTERIA
BIOASSAY
BIOLOGICAL WARFARE
BIOLOGICAL WARFARE AGENTS
CHEMICAL REACTIONS
DETECTION
DEVELOPED COUNTRIES
DNA
FEASIBILITY STUDIES
MICROORGANISMS
NATIONAL ORGANIZATIONS
NORTH AMERICA
NUCLEIC ACIDS
ORGANIC COMPOUNDS
POLYMERIZATION
STAPHYLOCOCCUS
US DOD
US ORGANIZATIONS
USA
WARFARE
WEAPONS
450600 -- Military Technology
Weaponry
& National Defense-- Chemical & Biological-- (1990)
560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BACILLUS
BACTERIA
BIOASSAY
BIOLOGICAL WARFARE
BIOLOGICAL WARFARE AGENTS
CHEMICAL REACTIONS
DETECTION
DEVELOPED COUNTRIES
DNA
FEASIBILITY STUDIES
MICROORGANISMS
NATIONAL ORGANIZATIONS
NORTH AMERICA
NUCLEIC ACIDS
ORGANIC COMPOUNDS
POLYMERIZATION
STAPHYLOCOCCUS
US DOD
US ORGANIZATIONS
USA
WARFARE
WEAPONS