T-DNA and opine synthetic loci in tumors incited by Agrobacterium tumefaciens A281 on soybean and alfalfa plants
A report is given of the molecular characterization of transferred DNA (T-DNA) in leguminous tumors incited by Agrobacterium tumefaciens A281 harboring the tumor-inducing plasmid pTiBo542. The T-DNA is composed of two regions named T/sub L/ (left portion)-DNA and T/sub R/ (right portion)-DNA, in accordance with the nomenclature for the octopine strains. T/sub L/-DNA is defined by several internal HindIII restriction fragments totaling 10.8 kilobase pairs (kbp) in uncloned soybean and alfalfa tumors. Alfalfa tumor DNA may contain one more HindIII fragment at the left end of T/sub L/-DNA that does soybean tumor DNA. T/sub R/-DNA has a 5.8-kbp BamHI-EcoRi internal fragment. All borders other than the left border of T/sub L/-DNA appear to be the same within the detection limits of Southern blot hybridization experiments. The two T-DNA regions are separated by 16 to 19 kbp of DNA not stably maintained in tumors. The distance from the left border of T/sub L/-DNA to the right border of T/sub R/-DNA is approximately 40 kbp. Loci for the mannityl opines are situated in T/sub R/-DNA, based on genetic and biochemical criteria.
- Research Organization:
- Washington Univ., St. Louis, MO
- DOE Contract Number:
- AC02-81ER10888
- OSTI ID:
- 6942559
- Journal Information:
- J. Bacteriol.; (United States), Journal Name: J. Bacteriol.; (United States) Vol. 168:3; ISSN JOBAA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550700 -- Microbiology
59 BASIC BIOLOGICAL SCIENCES
ALFALFA
BACTERIA
CELL CONSTITUENTS
CELL TRANSFORMATIONS
DISEASES
DNA
GENE REGULATION
GENES
GENETIC MAPPING
GLYCINE HISPIDA
LEGUMINOSAE
MAPPING
MICROORGANISMS
MOLECULAR STRUCTURE
NEOPLASMS
NUCLEIC ACIDS
ONCOGENIC TRANSFORMATIONS
ORGANIC COMPOUNDS
PATHOGENS
PLANTS
PLASMIDS
RECOMBINANT DNA
RHIZOBIUM
VIRULENCE