Transport of nonmetabolizable opines by Agrobacterium tumefaciens

Journal Article · Mon Dec 31 23:00:00 EST 1990 · Journal of Bacteriology; (USA)

OSTI ID:5988092

Krishnan, M; Burgner, J W; Chilton, W S; Gelvin, S B ^[1]

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1. Purdue Univ., West Lafayette, IN (USA)

We have examined the uptake of ({sup 14}C)octopine and ({sup 14}C)nopaline by Agrobacterium tumefaciens strains containing the C58 chromosomal background in medium suitable for the induction of vir genes. All strains tested could transport both of these opines, regardless of the presence or type of Ti plasmid (octopine or nopaline) present in the bacterium. The transport of these opines required active cellular metabolism. Nonradioactive octopine, nopaline, and arginine competed effectively with ({sup 14}C)octopine and ({sup 14}C)nopaline for transport into A. tumefaciens A136, suggesting that the transport of these opines occurs via an arginine transport pathway not encoded by the Ti plasmid.

• OSTI ID: 5988092
• Journal Information: Journal of Bacteriology; (USA), Journal Name: Journal of Bacteriology; (USA) Vol. 173:2; ISSN 0021-9193; ISSN JOBAA
• Country of Publication: United States
• Language: English

Related Subjects

550501* -- Metabolism-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
BACTERIA
BIOLOGICAL PATHWAYS
CARBON 14 COMPOUNDS
CARBOXYLIC ACIDS
CELL CONSTITUENTS
GENES
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MEMBRANE TRANSPORT
METABOLISM
MICROORGANISMS
ORGANIC ACIDS
ORGANIC COMPOUNDS
PLASMIDS
TRACER TECHNIQUES