Development and use of a new perfusion technique to study glucose metabolism of the aortic wall in normal and alloxan-diabetic rabbits

Thesis/Dissertation · Tue Jan 01 00:00:00 EST 1985

OSTI ID:6942076

Brown, B J.M.

This study investigated (1) possible alterations in glucose uptake and utilization in the perfused, normal, and diabetic vascular wall of rabbits and (2) the effects thereon of insulin and exogenous glucose concentration. Part I involved development and characterization of an in vitro perfusion technique that closely reproduced predetermined in vivo conditions of aortic blood flow, arterial blood pressure, heart rate and pulse pressure. The responsiveness of the preparation to vasoactive agents was assessed with concentrations of norepinephrine (NE) from 10/sup -9/ to 10/sup -4/ M. In Part II, the effects of NE-induced tension development on glucose metabolism were determined by perfusion with oxygenated physiological salt solution (PSS) containing 7 mM glucose and tracer amounts of uniformly labeled /sup 14/C-glucose. Aortas from 8 week-diabetic rabbits were perfused under similar conditions employing a NE infusion in the presence or absence of insulin (150 uU/ml) and variable levels of glucose. Effects of NE-induced tension development include an apparent increase (39%) in glucose uptake and a twofold increase in /sup 14/CO/sub 2/ and lactate production. Aortas from diabetic rabbits perfused with PSS containing 7 mM glucose demonstrated marked decreases in glucose uptake (74%), /sup 14/CO/sub 2/ (68%), lactate (30%), total tissue glycogen (75%) and labeled tissue phospholipids (70%). Insulin or elevation of exogenous glucose to 25 mM (diabetic levels) normalized glucose uptake, but had differential effects on the pattern of substrate utilization. The marked alterations of glucose metabolism in the diabetic state may contribute to the functional changes observed in diabetic blood vessels.

• Research Organization: Texas Tech Univ., Lubbock (USA)
• OSTI ID: 6942076
• Resource Relation: Other Information: Thesis (Ph. D.)
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
GLUCOSE
METABOLISM
UPTAKE
INSULIN
BIOLOGICAL EFFECTS
AORTA
CARBON 14 COMPOUNDS
DIABETES MELLITUS
IN VITRO
PERFUSED ORGANS
RABBITS
TRACER TECHNIQUES
ALDEHYDES
ANIMALS
ARTERIES
BLOOD VESSELS
BODY
CARBOHYDRATES
CARDIOVASCULAR SYSTEM
DISEASES
ENDOCRINE DISEASES
HEXOSES
HORMONES
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MAMMALS
METABOLIC DISEASES
MONOSACCHARIDES
ORGANIC COMPOUNDS
ORGANS
PEPTIDE HORMONES
SACCHARIDES
VERTEBRATES
550501* - Metabolism- Tracer Techniques