Determination of the frequency of several retroviral genomic elements in the cDNA from mouse testes

Journal Article · Sat Apr 01 04:00:00 EST 1989 · Journal of the Tennessee Academy of Science; (USA)

OSTI ID:6940949

Myer, F E; Chang, L Y; Yang, W K ^[1]; Byrne, T E

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1. Oak Ridge National Lab., TN (USA)

The purpose of this study is to determine the expression of the MRL class retroviral elements that may undergo frequent retrotransposition in the mouse germline, as suggested by the previous studies of this laboratory. A quantity of 300,000 plaque forming units of recombinant lambda phage containing mouse (Mus musculus) testes cDNA population were used to infect E. coli C 600 Hfl and subsequently were blotted onto nitrocellulose filters. Numbers of recombinant phage plaques containing retroviral genomic sequences were determined by hybridization of the nitrocellulose membrane filters with specific molecular probes representing the MRL elements and murine leukemia virus-related proviruses. From analysis of autoradiographs the observed mean numbers of positive clones per 10,000 plaque forming units were as determined. The results indicate relatively high content of MRL element transcripts in the mouse testis. Whether selective members or all the MRL multiple gene family in the mouse genome are involved in the expression remains to be investigated.

• DOE Contract Number: AC05-84OR21400
• OSTI ID: 6940949
• Journal Information: Journal of the Tennessee Academy of Science; (USA), Journal Name: Journal of the Tennessee Academy of Science; (USA) Vol. 64:2; ISSN 0040-313X; ISSN JTASA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
AUTORADIOGRAPHY
BACTERIA
BACTERIOPHAGES
BIOCHEMICAL REACTION KINETICS
BODY
DNA
ESCHERICHIA COLI
GONADS
HYBRIDIZATION
KINETICS
MALE GENITALS
MAMMALS
MICE
MICROORGANISMS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PARASITES
REACTION KINETICS
RECOMBINANT DNA
RODENTS
TESTES
VERTEBRATES
VIRUSES