Isolation and characterization of the human G sub s. alpha. gene

Journal Article · Fri Apr 01 04:00:00 EST 1988 · Proceedings of the National Academy of Sciences of the United States of America; (USA)

DOI:https://doi.org/10.1073/pnas.85.7.2081· OSTI ID:6940913

Kozasa, Tohru; Itoh, Hiroshi; Tsukamoto, Toshihiko; Kaziro, Yoshito ^[1]

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1. Univ. of Tokyo (Japan)

The gene for G{sub s}{alpha} (the {alpha} subunit of the guanine nucleotide-binding protein G{sub s}) was isolated from human genomic libraries using rat G{sub s}{alpha} cDNA as a probe. Comparison of the nucleotide sequence of the human gene with that of the rat cDNA revealed that the human G{sub s}{alpha} gene spans {approx}20 kilobases and is composed of 13 exons and 12 introns. Genomic Southern blot analysis suggests that the human haploid genome contains a single G{sub s}{alpha} gene. Previous reports indicated the presence of multiple species of G{sub s}{alpha} cDNA. The structure of the human G{sub s}{alpha} gene suggests that four types of G{sub s}{alpha} mRNAs may be generated from a single G{sub s}{alpha} gene by alternate use of exon 3 and/or of two 3{prime} splice sites of intron 3, where an unusual splice junction sequence (TG) instead of the consensus (AG) is used. S1 nuclease mapping analysis of human G{sub s}{alpha} mRNA identified multiple transcriptional initiation sites. The promoter region of the human G{sub s}{alpha} gene has extremely high G+C content (85%). It contains 4 GC boxes, but no typical TATA or CAAT box sequence. In the 5{prime} flanking region, there are several blocks of sequences that are similar to the sequences of the 5{prime} flanking region of the human cKi-ras2 gene.

• OSTI ID: 6940913
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (USA), Journal Name: Proceedings of the National Academy of Sciences of the United States of America; (USA) Vol. 85:7; ISSN 0027-8424; ISSN PNASA
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
ANIMALS
AUTORADIOGRAPHY
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
DAYS LIVING RADIOISOTOPES
DNA
DNA SEQUENCING
ELECTROPHORESIS
ENZYMES
ESTERASES
GENE REPRESSORS
GENES
HYBRIDIZATION
HYDROLASES
ISOTOPES
LIGHT NUCLEI
MAMMALS
MAN
MESSENGER-RNA
MOLECULAR STRUCTURE
NUCLEASES
NUCLEI
NUCLEIC ACIDS
NUCLEOPROTEINS
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PHOSPHODIESTERASES
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PRIMATES
PROTEINS
RADIOISOTOPES
RATS
RECOMBINANT DNA
RNA
RODENTS
STRUCTURAL CHEMICAL ANALYSIS
TRANSCRIPTION
VERTEBRATES